Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, Sichuan, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
BMC Vet Res. 2024 Nov 27;20(1):528. doi: 10.1186/s12917-024-04401-4.
Bacteroides fragilis is a prevalent anaerobic bacterium typically resides in the human vagina. It is known to potentially induce infections under specific conditions. Interestingly, there have been no previous reports of B. fragilis being isolated from the vagina of giant pandas.
A novel strain of anaerobic bacteria was isolated from the vaginal tract of a giant panda exhibiting symptoms of vaginitis. This strain, designated as GPBF01, was identified as Bacteroides fragilis, a species commonly found in the vaginal microbiome of humans and other animals. After purifying of the single colony, a series of evaluations were conducted including morphological examination, physiological and biochemical identification, antibiotic resistance analysis, resistance genes detection, 16S rRNA sequence, and phylogenetic tree sequence analysis to investigate its biological characteristics. The findings indicated the presence of a predominant anaerobic bacterium, which was identified as B. fragilis and temporarily named GPBF01 with unique biological traits not previously.
This study is the first to report B. fragilis in the vaginal tract of giant pandas. The analysis of antibiotic resistance patterns among anaerobic bacteria, as conducted in this research, is critical for informing the selection of appropriate antimicrobial agents in the clinical treatment of vaginitis in this species. The findings of this report substantially enhance the scientific basis needed to understand the etiology and refine therapeutic approaches for vaginitis in giant pandas.
脆弱拟杆菌是一种常见的厌氧细菌，通常存在于人类阴道中。已知在特定条件下它可能会引起感染。有趣的是，以前从未有过从大熊猫阴道中分离出脆弱拟杆菌的报道。
从一只患有阴道炎的大熊猫阴道中分离出一种新型厌氧细菌。该菌株被命名为 GPBF01，被鉴定为脆弱拟杆菌，这是一种常见于人类和其他动物阴道微生物群中的细菌。在纯化单个菌落后，进行了一系列评估，包括形态学检查、生理生化鉴定、抗生素耐药性分析、耐药基因检测、16S rRNA 序列和系统发育树序列分析，以研究其生物学特性。研究结果表明存在一种主要的厌氧细菌，被鉴定为脆弱拟杆菌，暂时命名为 GPBF01，具有以前从未有过的独特生物学特征。
本研究首次报道了大熊猫阴道中存在脆弱拟杆菌。本研究中进行的厌氧细菌抗生素耐药模式分析对于指导该物种阴道炎临床治疗中选择合适的抗菌药物至关重要。本报告的发现极大地增强了理解大熊猫阴道炎病因和完善治疗方法所需的科学基础。
