Department of Genetics and Biotechnology, University of Calabar, Calabar, Nigeria.
Department of Pure and Applied Botany, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.
BMC Res Notes. 2024 Nov 27;17(1):350. doi: 10.1186/s13104-024-07002-4.
The African bush apple (Heinsia crinita) is a highly valued orphan shrub that supports the nutritional and natural medicine needs of many sub-Saharan African communities. However, the crop remains poorly conserved and without any known genetic improvement. Accordingly, the current study sought to develop for the first time, an in vitro regeneration system based on direct shoot proliferation from its stem and hypocotyledonary explants using combinations of two cytokinins (benzyl adenine - BA, thidiazuron - TDZ) and the auxin (naphthalene acetic acid (NAA), in Murashige and Skoog (MS) medium.
Combinations of BA and NAA effectively induced multiple shoot formation from stem and hypocotyledonary explants of the crop. The most effective treatment (1.0 mg/L BA + 0.1 mg/L NAA) induced an average of 13.77 and 30.32 shoots per responsive hypocotyl and stem explants, respectively. Combinations of TDZ and NAA were less effective in promoting shoot induction in the explants at the concentrations tested compared to BA and NAA combinations. Hypocotyledonary explants achieved complete plant regeneration without multiple shoot formation in a hormone-free MS medium. In vitro shoots regenerated from both stem and hypocotyledonary explants were 100% successfully rooted on a half-strength hormone-free medium, and acclimatized to produce H. crinita plants with over 90.0% efficiency.
非洲布什苹果（Heinsia crinita）是一种非常有价值的孤儿灌木，它满足了许多撒哈拉以南非洲社区的营养和天然药物需求。然而，这种作物的保存状况仍然很差，也没有任何已知的遗传改良。因此，本研究首次试图建立一种基于茎和子叶外植体直接丛生芽增殖的离体再生系统，使用两种细胞分裂素（苄基腺嘌呤 - BA、噻二唑隆 - TDZ）和生长素（萘乙酸（NAA）组合，在 Murashige 和 Skoog（MS）培养基中。
BA 和 NAA 的组合有效地诱导了作物茎和子叶外植体的丛生芽形成。最有效的处理（1.0 mg/L BA + 0.1 mg/L NAA）诱导响应的下胚轴和茎外植体的平均每个分别产生 13.77 和 30.32 个芽。与 BA 和 NAA 组合相比，在测试浓度下，TDZ 和 NAA 的组合在促进外植体中芽诱导方面效果较差。子叶外植体在无激素的 MS 培养基中无需丛生芽形成即可实现完全的植株再生。从茎和子叶外植体再生的离体芽在半强度无激素培养基上 100%成功生根，适应环境后产生的 H. crinita 植物成活率超过 90.0%。
