Ortlund Eric, Hou Zhenxin, Chen Chih-Yu, Gaul David, Zhang Tiantian, Moore Samuel, Liu Xueyun, Ivanova Anna, Maner-Smith Kristal, Newgard Christopher, Bodine Sue, Savage Evan, Bennett Alexis, Fernandez Facundo
Emory University School of Medicine.
Emory University.
Res Sq. 2024 Nov 21:rs.3.rs-5263273. doi: 10.21203/rs.3.rs-5263273/v1.
Endurance exercise training (ExT) induces metabolic, structural, and functional adaptations via lipidomic modifications, yet the systematic elucidation of lipidome alterations in response to ExT remains incomplete. As a part of the Molecular Transducers of Physical Activity Consortium (MoTrPAC), we leveraged non-targeted and targeted lipidomics for the systematic discovery of lipid alterations in the brown adipose tissue, heart, hippocampus, kidney, liver, lung, skeletal muscle gastrocnemius, subcutaneous white adipose tissue, and plasma in response to 1, 2, 4 or 8 weeks of ExT in 6-month-old male and female Fischer-344 rats. This study demonstrates that these tissues, each with distinct lipidomic features, underwent dynamic, sexually dimorphic lipid remodeling. Exercise trained animals showed reduced whole-body adiposity and improved cardiorespiratory fitness, along with enhanced utilization of lipid stores and dynamic triacylglycerol remodeling compared to sedentary controls in all tissues except hippocampus. They also showed modifications in phospholipids, lysophospholipids, oxylipins, and ceramides in several tissues. Coordinated changes across tissues reflect systemic tissue communication, with liver-plasma-heart connection potentially playing a key role in systemic lipid metabolism during ExT. These data will improve our understanding of lipid-associated biological processes underlying the health-promoting benefits of ExT.
耐力运动训练（ExT）通过脂质组学修饰诱导代谢、结构和功能适应，但对ExT反应中脂质组变化的系统阐释仍不完整。作为体力活动分子传感器联盟（MoTrPAC）的一部分，我们利用非靶向和靶向脂质组学系统地发现了6月龄雄性和雌性Fischer-344大鼠在进行1、2、4或8周ExT后，其棕色脂肪组织、心脏、海马体、肾脏、肝脏、肺、骨骼肌腓肠肌、皮下白色脂肪组织和血浆中的脂质变化。这项研究表明，这些具有不同脂质组学特征的组织经历了动态的、性别二态性的脂质重塑。与久坐对照组相比，运动训练的动物全身脂肪减少，心肺功能改善，除海马体外，所有组织中脂质储存的利用率提高，三酰甘油动态重塑增强。它们在几个组织中的磷脂、溶血磷脂、氧化脂质和神经酰胺也有变化。组织间的协同变化反映了全身组织间的通讯，肝脏-血浆-心脏连接可能在ExT期间的全身脂质代谢中起关键作用。这些数据将增进我们对ExT促进健康益处背后脂质相关生物学过程的理解。
