University of Kansas Medical Center, Kansas City, Kansas, United States.
University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.
J Appl Physiol (1985). 2024 Sep 1;137(3):473-493. doi: 10.1152/japplphysiol.00102.2024. Epub 2024 Apr 18.
Physical activity, including structured exercise, is associated with favorable health-related chronic disease outcomes. Although there is evidence of various molecular pathways that affect these responses, a comprehensive molecular map of these molecular responses to exercise has not been developed. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is a multicenter study designed to isolate the effects of structured exercise training on the molecular mechanisms underlying the health benefits of exercise and physical activity. MoTrPAC contains both a preclinical and human component. The details of the human studies component of MoTrPAC that include the design and methods are presented here. The human studies contain both an adult and pediatric component. In the adult component, sedentary participants are randomized to 12 wk of Control, Endurance Exercise Training, or Resistance Exercise Training with outcomes measures completed before and following the 12 wk. The adult component also includes recruitment of highly active endurance-trained or resistance-trained participants who only complete measures once. A similar design is used for the pediatric component; however, only endurance exercise is examined. Phenotyping measures include weight, body composition, vital signs, cardiorespiratory fitness, muscular strength, physical activity and diet, and other questionnaires. Participants also complete an acute rest period (adults only) or exercise session (adults, pediatrics) with collection of biospecimens (blood only for pediatrics) to allow for examination of the molecular responses. The design and methods of MoTrPAC may inform other studies. Moreover, MoTrPAC will provide a repository of data that can be used broadly across the scientific community. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) will be the first large trial to isolate the effects of structured exercise training on the molecular mechanisms underlying the health benefits of exercise and physical activity. By generating a compendium of the molecular responses to exercise, MoTrPAC will lay the foundation for a new era of biomedical research on Precision Exercise Medicine. Presented here is the design, protocols, and procedures for the MoTrPAC human studies.
身体活动，包括有组织的运动，与有利的与健康相关的慢性病结果有关。虽然有证据表明各种分子途径会影响这些反应，但尚未开发出锻炼对这些分子反应的综合分子图谱。物理活动的分子转导者协会（MoTrPAC）是一项多中心研究，旨在分离有组织的运动训练对运动和身体活动带来的健康益处的分子机制的影响。MoTrPAC 既包含临床前部分，也包含人类部分。这里介绍了 MoTrPAC 人类研究部分的设计和方法。人类研究部分包括成人和儿科部分。在成人部分，久坐的参与者被随机分配到 12 周的对照组、耐力运动训练或阻力运动训练，在 12 周前后完成结果测量。成人部分还包括仅完成一次测量的高度活跃的耐力训练或阻力训练参与者的招募。儿科部分采用类似的设计；然而，仅检查耐力运动。表型测量包括体重、身体成分、生命体征、心肺健康、肌肉力量、身体活动和饮食以及其他问卷。参与者还完成一次急性休息期（仅成人）或运动期（成人、儿科），采集生物样本（儿科仅采集血液），以检查分子反应。MoTrPAC 的设计和方法可以为其他研究提供信息。此外，MoTrPAC 将提供一个可广泛用于科学界的数据存储库。物理活动的分子转导者协会（MoTrPAC）将是第一个分离有组织的运动训练对运动和身体活动带来的健康益处的分子机制的影响的大型试验。通过产生对运动的分子反应总览，MoTrPAC 将为精准运动医学的生物医学研究新时代奠定基础。这里介绍了 MoTrPAC 人类研究的设计、方案和程序。
