May Francis J, Baer Lisa A, Lehnig Adam C, So Kawai, Chen Emily Y, Gao Fei, Narain Niven R, Gushchina Liubov, Rose Aubrey, Doseff Andrea I, Kiebish Michael A, Goodyear Laurie J, Stanford Kristin I
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA 02215, USA.
Cell Rep. 2017 Feb 7;18(6):1558-1572. doi: 10.1016/j.celrep.2017.01.038.
Exercise improves whole-body metabolic health through adaptations to various tissues, including adipose tissue, but the effects of exercise training on the lipidome of white adipose tissue (WAT) and brown adipose tissue (BAT) are unknown. Here, we utilize MS/MS shotgun lipidomics to determine the molecular signatures of exercise-induced adaptations to subcutaneous WAT (scWAT) and BAT. Three weeks of exercise training decrease specific molecular species of phosphatidic acid (PA), phosphatidylcholines (PC), phosphatidylethanolamines (PE), and phosphatidylserines (PS) in scWAT and increase specific molecular species of PC and PE in BAT. Exercise also decreases most triacylglycerols (TAGs) in scWAT and BAT. In summary, exercise-induced changes to the scWAT and BAT lipidome are highly specific to certain molecular lipid species, indicating that changes in tissue lipid content reflect selective remodeling in scWAT and BAT of both phospholipids and glycerol lipids in response to exercise training, thus providing a comprehensive resource for future studies of lipid metabolism pathways.
运动通过对包括脂肪组织在内的各种组织产生适应性变化来改善全身代谢健康，但运动训练对白色脂肪组织（WAT）和棕色脂肪组织（BAT）脂质组的影响尚不清楚。在此，我们利用串联质谱鸟枪法脂质组学来确定运动诱导的对皮下白色脂肪组织（scWAT）和棕色脂肪组织（BAT）适应性变化的分子特征。为期三周的运动训练可降低scWAT中磷脂酸（PA）、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）的特定分子种类，并增加BAT中PC和PE的特定分子种类。运动还可降低scWAT和BAT中的大多数三酰甘油（TAGs）。总之，运动诱导的scWAT和BAT脂质组变化对某些分子脂质种类具有高度特异性，表明组织脂质含量的变化反映了scWAT和BAT中磷脂和甘油脂质对运动训练的选择性重塑，从而为未来脂质代谢途径的研究提供了全面的资源。
