Guo Min, Feng Xiaoli, Ma Jie, Zhu Kaichun, Niyazi Mayinuer
Department of Gynecology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China; Xinjiang Cervical Cancer Prevention and Treatment Clinical Research Center, China.
Department of Gynecology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China; Xinjiang Cervical Cancer Prevention and Treatment Clinical Research Center, China.
Microb Pathog. 2025 Jan;198:107149. doi: 10.1016/j.micpath.2024.107149. Epub 2024 Nov 26.
To analysis the differential composition in vaginal microbial communities following HPV infection in Uygur and Han women, and to Explore the correlation between these difference and the degree of Human Papillomavirus (HPV) infection in Uygur women compared to Han women.
A total of 151 Uygur and Han women, with and without HPV, were studied at Xinjiang Uygur Autonomous Region People's Hospital (June 2021-June 2022). These participants were divided into six groups: Uygur control (CV), Uygur transient infection (TPV), Uygur persistent infection (PPV), Han control (CH), Han transient infection (TPH), Han persistent infection (PPH). Vaginal microbiota diversity and dominant bacteria with or without HPV infection were compared using 16S rDNA sequencing.
After HPV infection, vaginal pH increased significantly in Uygur and Han women. Vaginal environment cleanliness decreased notably only in Han women (P < 0.05). In Han women, Lactobacillus and Gardnerella dominated both with or without HPV infection. Prevotella, Streptococcus, and Atopobium decreased, while Sneathia increased significantly in persistent HPV cases. Among Uygur women, Gardnerella, Streptococcus, Prevotella, and Shuttleworthia increased significantly in the TPV group, with lower Lactobacillus compared to CV and PPV groups. Bacterial diversity indices (Chao1, Shannon, Simpson) were significantly lower in TPH compared to CH (P < 0.05), with no significant difference in PPH compared to CH (P > 0.05). Chao1 index was significantly lower in TPH than TPV (P < 0.05), with no difference between PPH and PPV (P > 0.05). CV group's Chao1 index was significantly lower than CH (P < 0.01). PCoA and NMDS analyses showed distinct vaginal microbiota between TPH and TPV groups. LEfSe identified 20 differentially expressed taxa in CH-TPH-PPH comparison and 17 in CV-TPV-PPV.
Our study reveals ethnic-specific differences in vaginal microbial responses to HPV infection, with notable alterations in diversity and composition. The differential patterns observed between Uygur and Han women underscore the importance of considering host ethnicity in HPV-related microbial dysbiosis, emphasizing the need for tailored interventions targeting microbial signatures to enhance HPV prevention and management strategies.
分析维吾尔族和汉族女性感染人乳头瘤病毒（HPV）后阴道微生物群落的差异组成，探讨维吾尔族与汉族女性这些差异与HPV感染程度之间的相关性。
2021年6月至2022年6月期间，在新疆维吾尔自治区人民医院对151名有或无HPV感染的维吾尔族和汉族女性进行了研究。这些参与者被分为六组：维吾尔族对照组（CV）、维吾尔族短暂感染组（TPV）、维吾尔族持续感染组（PPV）、汉族对照组（CH）、汉族短暂感染组（TPH）、汉族持续感染组（PPH）。使用16S rDNA测序比较有无HPV感染时阴道微生物群的多样性和优势菌。
HPV感染后，维吾尔族和汉族女性的阴道pH值均显著升高。仅汉族女性的阴道环境清洁度显著下降（P<0.05）。在汉族女性中，无论有无HPV感染，乳酸杆菌和加德纳菌均占主导地位。在HPV持续感染病例中，普雷沃菌、链球菌和阿托波菌减少，而斯奈氏菌显著增加。在维吾尔族女性中，TPV组中的加德纳菌、链球菌、普雷沃菌和沙氏菌属显著增加，与CV组和PPV组相比，乳酸杆菌含量较低。与CH组相比，TPH组的细菌多样性指数（Chao1、香农、辛普森）显著较低（P<0.05），与CH组相比，PPH组无显著差异（P>0.05）。TPH组的Chao1指数显著低于TPV组（P<0.05），PPH组和PPV组之间无差异（P>0.05）。CV组的Chao1指数显著低于CH组（P<0.01）。主坐标分析（PCoA）和非度量多维尺度分析（NMDS）显示TPH组和TPV组的阴道微生物群不同。线性判别分析效应大小（LEfSe）在CH-TPH-PPH比较中鉴定出20个差异表达的分类群，在CV-TPV-PPV中鉴定出17个。
我们的研究揭示了HPV感染后阴道微生物反应的种族特异性差异，在多样性和组成方面有显著变化。维吾尔族和汉族女性之间观察到的差异模式强调了在HPV相关微生物失调中考虑宿主种族的重要性，强调了针对微生物特征进行定制干预以加强HPV预防和管理策略的必要性。
