LM1215 对 和 的影响。
Effects of LM1215 on and .
机构信息
Microbiome R&D Center, Lactomason, Seoul, Korea.
Center for Research and Development, Lactomason, Jinju, Korea.
出版信息
Yonsei Med J. 2024 Dec;65(12):727-740. doi: 10.3349/ymj.2023.0490.
PURPOSE
The aim of this study was to identify novel vaginal probiotics with the potential to prevent vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV).
MATERIALS AND METHODS
Eighteen strains of were isolated from healthy Korean women, and their antimicrobial effects against and were assessed. Three strains ( LM1203, LM1209, and LM1215) were selected for further investigation, focusing on their growth inhibition, biofilm regulation, and cellular mechanisms against these vaginal pathogens. Additionally, electron microscopy revealed damage to induced by LM1215, and genomic analysis was conducted on this strain.
RESULTS
LM1203, LM1209, and LM1215 showed approximately 1 and 2 Log CFU/mL growth reduction in and , respectively. These strains effectively inhibited biofilm formation and eliminated the mature biofilms formed by . Furthermore, LM1215 decreased tricarboxylic acid cycle activity by 51.75 (<0.001) and respiratory metabolic activity by 52.88% (<0.001) in . induced cellular membrane damage, inhibition of protein synthesis, and cell wall collapse in . Genomic analysis confirmed LM1215 as a safe strain for vaginal probiotics.
CONCLUSION
The LM1215 is considered a safe probiotic agent suitable for the prevention of VVC and BV.
目的
本研究旨在寻找具有预防阴道念珠菌病（VVC）和细菌性阴道病（BV）潜力的新型阴道益生菌。
材料和方法
从健康的韩国女性中分离出 18 株 ，评估其对 和 的抗菌作用。选择了 3 株（LM1203、LM1209 和 LM1215）进行进一步研究，重点研究其对这些阴道病原体的生长抑制、生物膜调节和细胞机制。此外，电子显微镜显示 LM1215 对 造成损伤，对该菌株进行了基因组分析。
结果
LM1203、LM1209 和 LM1215 对 和 分别有大约 1 和 2 Log CFU/mL 的生长减少。这些 菌株有效地抑制了生物膜的形成，并消除了 形成的成熟生物膜。此外，LM1215 使 的三羧酸循环活性降低了 51.75（<0.001），呼吸代谢活性降低了 52.88%（<0.001）。 诱导了 细胞膜损伤、蛋白质合成抑制和细胞壁塌陷。基因组分析证实 LM1215 是一种安全的阴道益生菌。
结论
LM1215 被认为是一种安全的益生菌剂，适合预防 VVC 和 BV。
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