Weng Shinuo, Hayashi Masaya, Inoue Yasuhiro, Wallingford John B
Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Department of Micro Engineering, Kyoto University, Nishikyo, Kyoto 615-8540, Japan.
Curr Biol. 2025 Jan 6;35(1):1-10.e3. doi: 10.1016/j.cub.2024.10.070. Epub 2024 Nov 27.
Convergent extension (CE) is an evolutionarily conserved developmental process that elongates tissues and organs via collective cell movements known as cell intercalation. Here, we sought to understand the mechanisms connecting cell behaviors and tissue shaping. We focus on an often-overlooked aspect of cell intercalation, the resolution of 4-cell vertices. Our data reveal that imbalanced cellular forces are involved in a timely vertex resolution, which, in turn, enables the propagation of such cellular forces, facilitating the propagation of tissue-scale CE. Conversely, delayed vertex resolution leads to a subtle but significant change in tissue-wide cell packing and exerts a profound impact by blocking force propagation, resulting in CE propagation defects. Our findings propose a collaborative nature of local cell intercalations in propagating tissue-wide CE. It unveils a multiscale biomechanical synergy underpinning the cellular mechanisms that orchestrate tissue morphogenesis during CE.
汇聚延伸（CE）是一种进化上保守的发育过程，它通过称为细胞插入的集体细胞运动来延长组织和器官。在这里，我们试图了解连接细胞行为和组织塑形的机制。我们关注细胞插入中一个经常被忽视的方面，即4细胞顶点的解析。我们的数据表明，不平衡的细胞力参与了及时的顶点解析，这反过来又使得这种细胞力得以传播，促进了组织尺度的CE传播。相反，延迟的顶点解析会导致全组织细胞堆积发生细微但显著的变化，并通过阻止力的传播产生深远影响，导致CE传播缺陷。我们的研究结果提出了局部细胞插入在传播全组织CE中的协同性质。它揭示了一种多尺度生物力学协同作用，这种协同作用支撑着在CE过程中协调组织形态发生的细胞机制。
