Liu Lan, Lian Zheng-Han, Lv Ai-Ping, Salam Nimaichand, Zhang Jian-Chao, Li Meng-Meng, Sun Wei-Min, Tan Sha, Luo Zhen-Hao, Gao Lei, Yuan Yang, Ming Yu-Zhen, OuYang Yu-Ting, Li Yu-Xian, Liu Ze-Tao, Hu Chao-Jian, Chen Ying, Hua Zheng-Shuang, Shu Wen-Sheng, Hedlund Brian P, Li Wen-Jun, Jiao Jian-Yu
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
National Agri-Food Biotechnology Institute, Mohali 140306, India.
Natl Sci Rev. 2024 Oct 23;11(11):nwae378. doi: 10.1093/nsr/nwae378. eCollection 2024 Nov.
Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway (RGP), Wood-Ljungdahl pathway (WLP) or Calvin-Benson-Bassham (CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms. Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP, whereas the WLP and CBB were acquired by horizontal gene transfer. Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon, sulfur and nitrogen. The name phy. nov. is proposed.
候选细菌门CSP1-3尚未被培养，人们对其了解甚少。在此，我们分析了112个CSP1-3宏基因组组装基因组，结果表明它们可能是兼性厌氧菌，5个科中有3个科通过还原型甘氨酸途径（RGP）、伍德-Ljungdahl途径（WLP）或卡尔文-本森-巴斯姆（CBB）以氢气或硫化物作为电子供体编码自养功能。来自温泉沉积物的化学自养富集培养物和荧光杂交显示有6个CSP1-3属得到富集，转录基因和DNA稳定同位素探测均与所提出的化学自养代谢一致。祖先状态重建表明，CSP1-3门的祖先可能是通过RGP进行自养的产乙酸菌，而WLP和CBB是通过水平基因转移获得的。我们的结果表明，CSP1-3是一个广泛分布的门，具有促进碳、硫和氮循环的潜力。建议命名为phy. nov.。
