Sangeetha Murthy, Sasirekha Sivakumar, Mahendran Jaganathan, Sivarajan Anbalmani, Radhakrishnan Manikkam, Manigundan Kaari, Dastager Syed G, Sengottuvelu Singaravel, Balagurunathan Ramasamy
Department of Microbiology, Periyar University, Salem, Tamil Nadu, India.
Kriya Biosys Pvt. Ltd., Salem, Tamil Nadu, India.
Int Microbiol. 2025 Aug;28(6):1269-1282. doi: 10.1007/s10123-024-00619-2. Epub 2024 Nov 29.
A pigmented bioactive molecule from Streptomyces kunmingensis BS19 was isolated, characterized, and evaluated for anti-infective, antiproliferative, and wound-healing properties. The yellow-red pigment produced from the strain BS19 showed promising activity against methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis. Extracellular bioactive pigment from the strain BS19 was produced by agar surface fermentation and purified through bioassay guided preparative HPLC-based purification. Based on the results of UV, FT-IR, GC-MS, H NMR, and C NMR spectral analyses, the purified pigment was identified as a chromopeptide class of molecule with phenoxazinone chromophore. Its molecular weight was determined as the chemical formula CHNO and molecular weight 1283 g/mol. It exhibited promising antimicrobial activity against Staphylococcus aureus (ATCC 1720) and antiproliferative activity against 14 types of human cancer cell lines. It showed good in vivo wound-healing activity in the rat model. The present study explored Streptomyces kunmingensis as a newly added source for the isolation of chromopeptide antibiotics for antimicrobial, wound-healing, and anticancer applications.
从昆明链霉菌BS19中分离出一种色素生物活性分子，对其进行了表征，并评估了其抗感染、抗增殖和伤口愈合特性。菌株BS19产生的黄红色色素对耐甲氧西林金黄色葡萄球菌（MRSA）和结核分枝杆菌显示出有前景的活性。菌株BS19的细胞外生物活性色素通过琼脂表面发酵产生，并通过生物测定引导的基于制备型高效液相色谱的纯化方法进行纯化。基于紫外光谱、傅里叶变换红外光谱、气相色谱-质谱联用、氢核磁共振光谱和碳核磁共振光谱分析结果，纯化后的色素被鉴定为具有吩恶嗪酮发色团的色肽类分子。其分子式确定为C₅₅H₈₁N₁₃O₁₆，分子量为1283 g/mol。它对金黄色葡萄球菌（ATCC 1720）表现出有前景的抗菌活性，对14种人类癌细胞系具有抗增殖活性。在大鼠模型中，它显示出良好的体内伤口愈合活性。本研究探索了昆明链霉菌作为分离用于抗菌、伤口愈合和抗癌应用的色肽抗生素的新增来源。
