Faculty of Biological Sciences, Department of Microbiology, Quaid-I-Azam University, Islamabad 45320, Pakistan.
Department of Cell Biology & Genetics, Shantou University Medical College, Shantou, PR China.
J Med Microbiol. 2024 Nov;73(11). doi: 10.1099/jmm.0.001936.
Obesity is a global health concern, affecting individuals of all ages and genders. One promising strategy to combat obesity is by addressing gut microbiota dysbiosis, with probiotics being a reliable intervention. However, single-strain probiotics may not effectively modulate the complex microbial communities in the gut, suggesting the need for multi-strain approaches. Probiotics are known to benefit gut health; however, the efficacy of single-strain probiotics in modulating gut microbiota is limited. Multi-strain probiotic community (MSPC) may offer a more effective approach for addressing obesity-related gut dysbiosis, but its specific effects on individuals and microbial diversity require further investigation. This study aimed to evaluate the potential of a dairy-origin MSPC in modulating obesity-related gut microbiota from lean and obese Pakistani volunteers using a simulated CoMiniGut model. Gut microbiota from lean and obese volunteers were treated with MSPC in a simulated CoMiniGut system. Bacterial counts, microbial diversity (- and β-diversity) and microbial community composition were analysed pre- and post-treatment. The impact of MSPC on specific bacterial genera and microbial metabolites was assessed, with statistical significance determined (≤0.05). The effect of MSPC was individualized, reducing bacterial counts in lean 1 and lean 2 samples, while significantly increasing bacterial counts in obese 2 and obese 3 samples (≤0.05). MSPC significantly improved -diversity in lean 2, lean 3, obese 2 and obese 3 samples (≤0.05). Proteobacteria decreased in the lean group and increased in the obese group post-MSPC treatment. In the lean group, pathogenic bacteria such as , and were significantly reduced (≤0.05), whereas beneficial bacteria like and increased significantly in the obese group (≤0.05). Among the selected metabolites, only butanoic acid was detected in all tested samples, with MSPC affecting metabolite concentrations and types. MSPC demonstrated a potential for modulating gut microbiota dysbiosis in both lean and obese individuals, with effects on bacterial counts, microbial diversity and metabolite concentrations. MSPC could serve as a promising option for personalized the modulation of gut microbiota in obesity management.
肥胖是一个全球性的健康问题，影响着各个年龄段和性别的人。一种有前途的对抗肥胖的策略是解决肠道微生物群落失调，益生菌是一种可靠的干预方法。然而，单一菌株益生菌可能无法有效地调节肠道中复杂的微生物群落，这表明需要采用多菌株方法。益生菌已知对肠道健康有益；然而，单一菌株益生菌调节肠道微生物群落的功效是有限的。多菌株益生菌群落（MSPC）可能为解决肥胖相关的肠道菌群失调提供更有效的方法，但它对个体和微生物多样性的具体影响需要进一步研究。本研究旨在使用模拟 CoMiniGut 模型，评估一种源自乳制品的 MSPC 调节来自巴基斯坦瘦人和肥胖志愿者的肥胖相关肠道微生物群落的潜力。使用模拟 CoMiniGut 系统，将瘦人和肥胖志愿者的肠道微生物群与 MSPC 一起处理。在处理前后分析细菌计数、微生物多样性（α-和β-多样性）和微生物群落组成。评估了 MSPC 对特定细菌属和微生物代谢物的影响，并确定了统计学意义（≤0.05）。MSPC 的影响是个体化的，降低了瘦人 1 和瘦人 2 样本中的细菌计数，而显著增加了肥胖 2 和肥胖 3 样本中的细菌计数（≤0.05）。MSPC 显著改善了瘦人 2、瘦人 3、肥胖 2 和肥胖 3 样本的 α-多样性（≤0.05）。厚壁菌门在瘦组中减少，在肥胖组中增加。在瘦组中，致病性细菌如 、 和 显著减少（≤0.05），而在肥胖组中有益细菌如 和 显著增加（≤0.05）。在所选择的代谢物中，只有丁酸在所有测试样本中都有检测到，MSPC 影响代谢物浓度和类型。MSPC 显示出在瘦人和肥胖个体中调节肠道微生物群落失调的潜力，对细菌计数、微生物多样性和代谢物浓度都有影响。MSPC 可能成为肥胖管理中个性化调节肠道微生物群落的有前途的选择。
