Guo Bingbing, Zhang Weihao, Zhang Jingyi, Zou Jingwen, Dong Ningning, Liu Bin
College of Chemistry and Life Science, Beijing University of Technology, Beijing, China.
Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University/National Clinical Research Center of Gastrointestinal Disease/Beijing Digestive Disease Center/Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing, China.
Food Res Int. 2025 Jan;199:115385. doi: 10.1016/j.foodres.2024.115385. Epub 2024 Nov 19.
Gut dysbiosis is a characteristic feature of obesity and targeting gut microbiota presents a promising approach to attenuate obesity. Euglena gracilis polysaccharide (EGP) has emerged as a potential prebiotic capable of promoting health-beneficial bacteria. However, its effects on the gut dysbiosis of obese individuals remain unclear. This study investigated the impacts of EGP on gut microbiota from both non-obese and obese individuals using an in vitro fermentation model. Results showed that EGP significantly altered the gut microbiota composition and metabolism. Specifically, EGP improved the relative abundance of Paeniclostridium, Clostridium_sensu_stricto_1 and Paraclostridium of the non-obese individuals and Providencia, Enterococcus and Bacteroides of the obese individuals. Metabolomics results showed EGP significantly altered the lipid metabolism especially in the obese group with enriched bile secretion and cholesterol metabolism pathways. Noting that acetic acid was significantly increased in both groups, these acetic acid favorable microbiota from non-obese individuals was collected with acetic acid supplementation. Transplantation of these acetic acid-induced microbiota (AAiM) notably improved the richness and diversity of fecal microbiota of the obese individuals, enhancing the growth of probiotics like Bacteroides and Bifidobacterium. Consequently, AAiM significantly restructured macronutrients (including amino acids, carbohydrates and lipids) metabolism of the gut microbiota from obese individuals. Altogether, this study underscores the potential of EGP and acetic acid favorable microbiota in manipulating obesity-associated gut dysbiosis via acetic acid production.
肠道菌群失调是肥胖的一个特征，针对肠道微生物群是减轻肥胖的一种有前景的方法。纤细裸藻多糖（EGP）已成为一种潜在的益生元，能够促进有益健康的细菌生长。然而，其对肥胖个体肠道菌群失调的影响仍不清楚。本研究使用体外发酵模型研究了EGP对非肥胖和肥胖个体肠道微生物群的影响。结果表明，EGP显著改变了肠道微生物群的组成和代谢。具体而言，EGP提高了非肥胖个体中假丁酸梭菌、严格梭菌属1和副梭菌以及肥胖个体中普罗威登斯菌、肠球菌和拟杆菌的相对丰度。代谢组学结果表明，EGP显著改变了脂质代谢，尤其是在肥胖组中，胆汁分泌和胆固醇代谢途径得到了富集。注意到两组中乙酸均显著增加，通过补充乙酸收集了来自非肥胖个体的这些有利于乙酸产生的微生物群。移植这些乙酸诱导的微生物群（AAiM）显著改善了肥胖个体粪便微生物群的丰富度和多样性，促进了拟杆菌和双歧杆菌等益生菌的生长。因此，AAiM显著重塑了肥胖个体肠道微生物群的大量营养素（包括氨基酸、碳水化合物和脂质）代谢。总之，本研究强调了EGP和有利于乙酸产生的微生物群通过产生乙酸来控制肥胖相关肠道菌群失调的潜力。
