Dubaybo B A, Thet L A
Res Commun Chem Pathol Pharmacol. 1986 Feb;51(2):211-20.
Lung lavage fibronectin is known to be elevated after experimental lung injury; however, changes in lung tissue fibronectin content probably more directly reflect tissue injury and repair. We injured rat lungs with a single injection of paraquat and serially measured the fibronectin content of both lung lavage fluid and lung tissue. Changes in lung ultrastructural morphology and collagen content were also assessed. Although both lavage and tissue content of fibronectin increased, the temporal patterns of increase were very different. Lavage fibronectin peaked at 1 day post-injury, was normal by 7 days, and paralleled changes in lavage albumin. Tissue fibronectin was normal at 1 day post-injury, peaked at 4 days and was still high at 7 and 14 days. The period of increased tissue fibronectin coincided with electron microscopic evidence of cell injury and repair at 7 days. By 14 days post-injury the lungs appeared virtually normal. Lung collagen content remained normal throughout the period of injury and repair. We conclude that increases in lung lavage fibronectin do not necessarily correlate with changes in lung tissue fibronectin and that the latter may more accurately reflect lung tissue repair. In addition, even large increases in both measurements do not necessarily predict the development of post-injury fibrosis.
已知实验性肺损伤后肺灌洗纤连蛋白会升高；然而，肺组织纤连蛋白含量的变化可能更直接地反映组织损伤和修复情况。我们通过单次注射百草枯损伤大鼠肺部，并连续测量肺灌洗 fluid 和肺组织中的纤连蛋白含量。还评估了肺超微结构形态和胶原蛋白含量的变化。虽然灌洗和组织中的纤连蛋白含量均增加，但增加的时间模式非常不同。灌洗纤连蛋白在损伤后 1 天达到峰值，7 天时恢复正常，且与灌洗白蛋白的变化平行。组织纤连蛋白在损伤后 1 天正常，4 天达到峰值，7 天和 14 天时仍较高。组织纤连蛋白增加的时期与 7 天时细胞损伤和修复 的电子显微镜证据一致。损伤后 14 天时，肺几乎恢复正常。在整个损伤和修复期间，肺胶原蛋白含量保持正常。我们得出结论，肺灌洗纤连蛋白的增加不一定与肺组织纤连蛋白的变化相关，后者可能更准确地反映肺组织修复。此外，即使这两项测量值都大幅增加，也不一定能预测损伤后纤维化的发展。
