Production, extraction, and authentication of natural and non-natural vanillin. A comprehensive review and economic future biotechnology perspectives.

Vanillin is a chief flavoring agent owing to its immense popularity in food, beverage, and pharmaceutical industries. This study holistically dissects vanillin quality control approaches that include conventional, hyphenated, and sensory analyses. Markers to differentiate between authentic, synthetic, and adulterated vanilla are highlighted using hyphenated techniques. Carbon isotope ratio range appears of potential to identify vanillin originating from biosynthetic (C3 plant), synthetic (petroleum) sources, or vanilla pods. Novel extraction methods typically provide greater selectivity, higher purity, shorter extraction times, and ecofriendly attributes compared to conventional methods. Best methods include supercritical fluids (SCF) or natural deep eutectic solvents (NADES) that promoted higher yield of vanillin. The review also highlights the promising avenue of biotransformation, the safest technique for the production of vanilla flavor components, tackling current challenges and emphasizing its potential to meet the market needs for authenticated and high-quality yields of vanillin.