Mukherjee Ratnadeep, Eggesbø Linn Margrethe, Wolf Asia-Sophia, Kjønstad Ingrid Fadum, Solum Guri, Ravussin Anthony, Bhandari Sabin, Robertson Anna Hayman, Magnus Per, Trogstad Lill, Kristoffersen Anja Bråthen, Nygaard Unni Cecilie, Mjaaland Siri
Division of Infection Control, Section for Immunology, Norwegian Institute of Public Health, Oslo, Norway.
Division of Infection Control, Section for Vaccine Epidemiology and Population Studies, Norwegian Institute of Public Health, Oslo, Norway.
NPJ Vaccines. 2024 Nov 29;9(1):238. doi: 10.1038/s41541-024-01028-2.
Heterogeneity in vaccine response, particularly in vulnerable populations like the elderly, represents a significant public health challenge. We conducted an in-depth examination of immune cell profiles before and after SARS-CoV-2 vaccination utilizing mass cytometry in a cohort of healthy Norwegian seniors (65-80 years). We have demonstrated that higher pre-vaccination frequencies of CD27IgD class-switched memory B cells and subsets of CD27CD24CD38 transitional B cells were associated with a robust vaccine response. Post-vaccination, high responders exhibited increased frequencies of IFN-γCD4 T cells with antigen recall and a concurrent decrease in CCR6(+) T cell subset frequencies compared to low responders. The presence of a γδ T cell subset displaying polyfunctional cytokine responses was also associated with better vaccine response in the elderly. This in-depth profiling sheds light on inherent differences in immune cell frequencies and functions that may offer insights for targeted vaccination strategies in older populations.
疫苗反应的异质性，尤其是在老年人等弱势群体中，是一项重大的公共卫生挑战。我们利用质谱流式细胞术对一组健康的挪威老年人（65 - 80岁）在接种SARS-CoV-2疫苗前后的免疫细胞谱进行了深入研究。我们已经证明，接种疫苗前较高频率的CD27IgD类别转换记忆B细胞和CD27CD24CD38过渡性B细胞亚群与强劲的疫苗反应相关。接种疫苗后，与低反应者相比，高反应者在抗原回忆时IFN-γ CD4 T细胞频率增加，同时CCR6(+) T细胞亚群频率下降。显示多功能细胞因子反应的γδ T细胞亚群的存在也与老年人更好的疫苗反应相关。这种深入分析揭示了免疫细胞频率和功能的内在差异，可能为老年人群的靶向疫苗接种策略提供见解。
