He Shilin, Zhang Yanling
Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University, No. 3 East Qingchun Road, Hangzhou 310016, China; Zhejiang Province Medical Research Center of Minimally Invasive Diagnosis and Treatment of Abdominal Diseases, Hangzhou, China; National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments, Hangzhou, China.
Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Reprod Toxicol. 2025 Mar;132:108757. doi: 10.1016/j.reprotox.2024.108757. Epub 2024 Nov 29.
With the increasing use of plastics, microplastic (MPs) pollution has garnered significant attention in recent years. Endometrial polyps are prevalent gynecological conditions in women of childbearing age, which impair endometrial receptivity and contribute to female infertility. However, no studies have yet reported the exposure of endometrial polyps to MPs. This study employed pyrolysis-gas chromatography/mass spectrometry and laser direct infrared spectroscopy to detect and compare MPs between normal endometrium and endometrial polyps. Using Py-GC/MS, we identified three main MPs in 14 normal endometrial samples and 16 endometrial polyps. The average abundance of MPs in the endometrial polyp group was significantly higher than in the normal endometrium group. The respective average abundance of polyethylene (PE), polystyrene (PS), and polyvinyl chloride (PVC) in the polyp and normal endometrium groups was 13.66 ± 2.0 vs. 7.132 ± 0.78 μg/g (p = 0.0009), 94.81 ± 10.67 vs. 69.29 ± 6.93 μg/g, and 67.67 ± 11.02 vs. 56.35 ± 6.90 μg/g. LDIR analysis revealed 13 different types of MPs, with polymethylmethacrylate being the most prevalent. Moreover, we discovered that PS microspheres can promote the proliferation and migration of endometrial stromal cells through PI3K/AKT pathway, which may be a key factor in the formation of endometrial polyps. This study is the first to explore the presence of MPs in endometrial polyps, compare the differences in MPs content between normal endometrium and endometrial polyps, and clarify the potential connection between MPs exposure and the formation of endometrial polyps. Further research is required to explore additional potential insights.
随着塑料使用的增加，微塑料（MPs）污染近年来受到了广泛关注。子宫内膜息肉是育龄期女性常见的妇科疾病，它会损害子宫内膜容受性并导致女性不孕。然而，尚无研究报道过子宫内膜息肉与微塑料的接触情况。本研究采用热解气相色谱/质谱联用技术和激光直接红外光谱法检测并比较正常子宫内膜和子宫内膜息肉中的微塑料。通过热解气相色谱/质谱联用技术，我们在14个正常子宫内膜样本和16个子宫内膜息肉样本中鉴定出了三种主要的微塑料。子宫内膜息肉组中微塑料的平均丰度显著高于正常子宫内膜组。息肉组和正常子宫内膜组中聚乙烯（PE）、聚苯乙烯（PS）和聚氯乙烯（PVC）的各自平均丰度分别为13.66±2.0 vs. 7.132±0.78μg/g（p = 0.0009）、94.81±10.67 vs. 69.29±6.93μg/g以及67.67±11.02 vs. 56.35±6.90μg/g。激光直接红外光谱分析揭示了13种不同类型的微塑料，其中聚甲基丙烯酸甲酯最为常见。此外，我们发现聚苯乙烯微球可通过PI3K/AKT途径促进子宫内膜基质细胞的增殖和迁移，这可能是子宫内膜息肉形成的关键因素。本研究首次探索了子宫内膜息肉中微塑料的存在情况，比较了正常子宫内膜和子宫内膜息肉中微塑料含量的差异，并阐明了微塑料暴露与子宫内膜息肉形成之间的潜在联系。还需要进一步研究以探索更多潜在的见解。
