Henan Office for Cancer Control and Research, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital. Henan International Joint Laboratory of Cancer Prevention, Henan Engineering Research Center of Cancer Prevention and Control, 450008 Zhengzhou, Henan, China; College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Hematology Department, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 450008 Zhengzhou, Henan, China.
J Hazard Mater. 2024 Sep 15;477:135266. doi: 10.1016/j.jhazmat.2024.135266. Epub 2024 Jul 20.
The health implications of human exposure to microplastics (MPs) have raised significant concerns. While evidence indicates MPs can accumulate in closed human organs like the heart, placenta, and blood, there is no available data on MP exposure specifically within the human bone marrow. To fill the research gap, this study detected the concentration of microplastics (MPs) in bone marrow samples by pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and assessed the size range and morphological characteristics of MPs by Laser Direct Infrared Spectroscopy (LD-IR) and scanning electron microscopy (SEM). Our study shows that MPs were present in all 16 bone marrow samples, with an average concentration of 51.29 µg/g ranging from 15.37 µg/g to 92.05 µg/g. Five polymer types-polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC), polyadiohexylenediamine 66 (PA66), and polypropylene (PP), were identified. PE was the most frequent polymer detected in the bone marrow, with an average concentration of 30.02 µg/g ranging from 14.77 µg/g to 52.57 µg/g, with a detection rate of 93.75 %. PS had the highest detection rate at 100 % of bone marrow samples, while PVC and PA66 were found in 75 % of samples each. LD-IR analysis revealed the identification of 25 polymer types, with an average abundance of 19.72 particles/g. Of these, 89.82 % of the MPs were smaller than 100 µm. In summary, this study has, for the first time, demonstrated the presence of MPs are deeply embedded within human bone marrow, providing a basis for future investigations into their potential toxicological effects and underlying mechanisms affecting the hematopoietic system.
人体接触微塑料（MPs）对健康的影响引起了人们的高度关注。虽然有证据表明 MPs 可以在心脏、胎盘和血液等封闭的人体器官中积累，但目前还没有关于 MPs 暴露于人体骨髓的具体数据。为了填补这一研究空白，本研究通过热裂解气相色谱-质谱联用技术（Py-GC/MS）检测了骨髓样本中微塑料（MPs）的浓度，并通过激光直接红外光谱（LD-IR）和扫描电子显微镜（SEM）评估了 MPs 的粒径范围和形态特征。我们的研究表明，所有 16 个骨髓样本中均存在 MPs，平均浓度为 51.29µg/g，范围为 15.37µg/g 至 92.05µg/g。共鉴定出 5 种聚合物类型：聚乙烯（PE）、聚苯乙烯（PS）、聚氯乙烯（PVC）、聚己二酰己二胺 66（PA66）和聚丙烯（PP）。PE 是骨髓中最常见的聚合物，平均浓度为 30.02µg/g，范围为 14.77µg/g 至 52.57µg/g，检出率为 93.75%。PS 的检出率最高，为 100%，而 PVC 和 PA66 的检出率各为 75%。LD-IR 分析显示共鉴定出 25 种聚合物类型，平均丰度为 19.72 个/克。其中，89.82%的 MPs 粒径小于 100µm。综上所述，本研究首次证明 MPs 深深嵌入人体骨髓中，为未来研究其潜在的毒理学效应和影响造血系统的潜在机制提供了依据。
