Liu Yixuan, Berrada Houda, Castagnini Juan M, Barba Francisco J
Research group in Innovative Technologies for Sustainable Food (ALISOST), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100, Burjassot, València, Spain.
Research group in Innovative Technologies for Sustainable Food (ALISOST), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100, Burjassot, València, Spain; Alternative methods for the determination of toxic effects and risk assessment of contaminants and mixtures (RISKTOX), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100, Burjassot, València, Spain.
Food Chem. 2025 Mar 1;467:142283. doi: 10.1016/j.foodchem.2024.142283. Epub 2024 Nov 29.
A sequential extraction process combining supercritical fluid extraction (SFE: 25.0 MPa, 75 min, 50.0 °C, and 10.0 mL/min flow) with pulsed electric fields (PEF: 3.0 kV/cm, 300 kJ/kg for head/backbones, 124.8 kJ/kg for viscera) or conventional extraction (CE: 60 min, 300 rpm) was optimized to enhance the recovery of high-added-value compounds, including proteins, bioactive peptides, minerals, heavy metals, and total antioxidant capacity (TAC) from salmon side streams. Protein levels increased notably with PEF, particularly in viscera liquid extracts, reaching 15.5 g/100 g. Bioactive peptides displayed anti-inflammatory, immunomodulatory, anticancer, hypolipidemic, and antithrombotic potential. Viscera liquid extracts showed high TAC (402.8 and 3263.6 μM). ICP-MS analysis revealed PEF treatment helps solid matrices retaining more minerals and heavy metals. Principal component analysis (PCA) on proteins, peptides, minerals, and heavy metals elucidated PEF's impact, identifying two main components affecting the salmon matrix and extract composition.
一种将超临界流体萃取（SFE：25.0兆帕，75分钟，50.0℃，流速10.0毫升/分钟）与脉冲电场（PEF：3.0千伏/厘米，头部/主干300千焦/千克，内脏124.8千焦/千克）或传统萃取（CE：60分钟，300转/分钟）相结合的顺序萃取工艺，已得到优化，以提高从鲑鱼副产物中回收高附加值化合物的效率，这些化合物包括蛋白质、生物活性肽、矿物质、重金属和总抗氧化能力（TAC）。蛋白质含量在PEF处理后显著增加，尤其是在内脏液体提取物中，达到15.5克/100克。生物活性肽具有抗炎、免疫调节、抗癌、降血脂和抗血栓形成的潜力。内脏液体提取物显示出较高的TAC（402.8和3263.6微摩尔）。电感耦合等离子体质谱（ICP-MS）分析表明，PEF处理有助于固体基质保留更多的矿物质和重金属。对蛋白质、肽、矿物质和重金属进行主成分分析（PCA），阐明了PEF的影响，确定了影响鲑鱼基质和提取物组成的两个主要成分。
