Jani Twisha, Santoro Domenico, Shmalberg Justin
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16(th) Ave., Gainesville, FL 32610, USA.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16(th) Ave., Gainesville, FL 32610, USA.
Res Vet Sci. 2025 Jan;182:105483. doi: 10.1016/j.rvsc.2024.105483. Epub 2024 Nov 29.
Cannabidiol (CBD) has been shown to have anti-inflammatory and antipruritic properties without the significant psychoactive effects. This study aims to evaluate the cytotoxic effects of, and the production of cytokines after exposure to CBD, cannabidiolic acid (CBDA), and β-caryophyllene (BCP), alone and in combination, by peripheral blood mononuclear cells (PBMC) from healthy and atopic dogs. Six healthy and five atopic, privately-owned dogs were enrolled. Peripheral blood mononuclear cells were harvested and incubated for 24 h with different concentrations of CBD, CBDA, and BCP alone or in combination. Cell viability and inflammatory cytokines were assessed. There was no difference in cell viability between baseline and tested concentrations of CBD, CBDA, or BCP in either healthy or in atopic PBMC. There was no effect of CBD, CBDA and BCP on the secretion of cytokines compared to baseline in healthy or atopic PMBC. The only exception was interleukin (IL)-10, increased in healthy PMBC exposed to CBD 100 ng/mL (p = 0.031) or CBDA 600 ng/mL (p = 0.017). Tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP-1), IL-2, and IL-18 were higher in atopic PBMC exposed to combinations of CBD, CBDA, and BCP compared to healthy post-exposure PBMC. This is the first study that tested the effect of CBD, CBDA, and BCP at different concentrations on atopic and healthy canine PBMC. The results of this study show that CBD, CBDA and BCP, at the tested concentrations, are safe for canine PBMC. However, CBD, CBDA and BCP did not show any direct anti-inflammatory effect under these experimental conditions. Further research is needed to confirm these results in a larger canine population.
大麻二酚（CBD）已被证明具有抗炎和止痒特性，且无明显的精神活性作用。本研究旨在评估健康犬和特应性犬的外周血单个核细胞（PBMC）单独或联合暴露于CBD、大麻二酚酸（CBDA）和β-石竹烯（BCP）后的细胞毒性作用以及细胞因子的产生。招募了6只健康的和5只特应性的私人拥有犬。采集外周血单个核细胞，并分别与不同浓度的CBD、CBDA和BCP单独或联合孵育24小时。评估细胞活力和炎性细胞因子。在健康或特应性PBMC中，CBD、CBDA或BCP的基线浓度与测试浓度之间的细胞活力没有差异。与健康或特应性PBMC的基线相比，CBD、CBDA和BCP对细胞因子的分泌没有影响。唯一的例外是白细胞介素（IL）-10，在暴露于100 ng/mL CBD（p = 0.031）或600 ng/mL CBDA（p = 0.017）的健康PBMC中升高。与暴露后健康的PBMC相比，暴露于CBD、CBDA和BCP组合的特应性PBMC中的肿瘤坏死因子（TNF）-α、单核细胞趋化蛋白（MCP）-1、IL-2和IL-18更高。这是第一项测试不同浓度的CBD、CBDA和BCP对特应性和健康犬PBMC作用的研究。本研究结果表明，在测试浓度下，CBD、CBDA和BCP对犬PBMC是安全的。然而，在这些实验条件下，CBD、CBDA和BCP未显示出任何直接的抗炎作用。需要进一步研究以在更大的犬群中证实这些结果。
