青钱柳多糖对糖尿病大鼠肝损伤的抗氧化及免疫作用
Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats.
作者信息
Xichao Xia, Shipeng Xue, Guoying Song, Bin L I, Huiping Wang, Ju Qiu, Jihong Wang, Qingchun Liu, Yuhong M A, Jingfeng Ouyang
机构信息
Nursing College, Pingdingshan University, Pingdingshan 467000, China.
Basic Medicine College of Nanyang Medical University, Nanyang 473041, China.
出版信息
J Tradit Chin Med. 2024 Dec;44(6):1146-1152. doi: 10.19852/j.cnki.jtcm.2024.06.005.
OBJECTIVE
To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharide on the liver injury of diabetic rats.
METHODS
Rats were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharide treatment, middle-dose group of C. paliurus polysaccharide treatment and high-dose group of C. paliurus polysaccharide treatment. Histological analysis of liver was analyzed using hematoxilin and eosin. Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Nuclear factor kappa-B-p65 (NF-κB p65), tumor necrosis factor-α (TNF-α), interleukins 6 (IL-6), IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.
RESULTS
Compared with that of model group, the glucose level of plasma decreased 62.32% ( < 0.01), but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C. paliurus polysaccharide treatment, respectively. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) level decreased 47.47% ( 0.05), 43.65% ( 0.05) and 50.51% ( 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 1.16 times ( 0.01), 71.28% ( 0.05), 1.29 times ( 0.01) and 87.46% ( 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Enzyme activities of NF-κB, TNF-α, IL-1β and IL-6 were decreased 39.29% ( 0.05), 51.11% ( < 0.05), 37.42% ( < 0.05) and 36.50% ( 0.05), respectively.
CONCLUSIONS
Administration of C. paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose, ALT, AST, ALP level, increasing glycogen and insulin level, enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.
目的
探讨青钱柳多糖对糖尿病大鼠肝损伤的影响。
方法
将大鼠分为6组，包括正常组、模型组、对照组、青钱柳多糖低剂量治疗组、青钱柳多糖中剂量治疗组和青钱柳多糖高剂量治疗组。采用苏木精-伊红染色对肝脏进行组织学分析。用分光光度法测定血浆生物学参数和抗氧化酶水平。通过实时聚合酶链反应和酶联免疫吸附测定法检测核因子κB-p65（NF-κB p65）、肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）、IL-1β。
结果
与模型组相比，青钱柳多糖高剂量治疗组血浆葡萄糖水平降低62.32%（P<0.01），肝糖原和胰岛素水平分别升高1.51倍和1.27倍。青钱柳多糖高剂量治疗组血浆丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）水平分别降低47.47%（P<0.05）、43.65%（P<0.05）和50.51%（P<0.05）。青钱柳多糖高剂量治疗组超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶水平分别升高1.16倍（P<0.01）、71.28%（P<0.05）、1.29倍（P<0.01）和87.46%（P<0.05）。NF-κB、TNF-α、IL-1β和IL-6的酶活性分别降低39.29%（P<0.05）、51.11%（P<0.05）、37.42%（P<0.05）和36.50%（P<0.05）。
结论
给予青钱柳多糖可能通过降低血糖、ALT、AST、ALP水平，升高肝糖原和胰岛素水平，增强抗氧化能力及下调炎症因子表达，对糖尿病大鼠肝损伤起到保护作用。
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