Nasirian Fariba, Dadkhah Masoumeh, Moradi-Kor Nasrollah, Obeidavi Zia
Department of Animal Sciences, University of Birjand, Birjand, Iran.
Research Centers Development and Coordination Office, Deputy of Research & Technology, Ardabil University of Medical Sciences, Ardabil, Iran.
Diabetes Metab Syndr Obes. 2018 Jul 31;11:375-380. doi: 10.2147/DMSO.S172104. eCollection 2018.
Lipid peroxidation and hyperglycemia are common signs for diabetes. Natural antioxidants such as microalgae (SPM) may prevent lipid peroxidation and hyperglycemia. This study aimed to evaluate the effects of SPM on antioxidant and anti-inflammatory in diabetic rats.
Sixty-four rats were divided into eight groups (n=8) and orally treated with 0, 10, 20 and 30 mg/kg body weight of SPM extract. Experimental groups included diabetic rats fed with 0 (DC), 10, 20 and 30 mg/kg SPM. Healthy rats were treated with 0 mg/kg SPM (HC), 10 mg/kg SPM, 20 mg/kg SPM and 30 mg/kg SPM. At the end of the trial, blood samples were collected and the plasma concentrations of trace minerals (TMs), biochemical parameters, and antioxidant enzymes in liver were evaluated. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), TNF-α (tumor necrosis factor-alpha) and IL-6 (interleukin-6) were evaluated.
Our findings showed that diabetes significantly lowered the plasma concentration of TMs and antioxidant enzymes in liver and also increased the levels of malondialdehyde, glucose, lipid profile, AST, ALT, TNF-α and IL-6 (DC vs HC). However, an oral supplement of SPM (20 and 30 mg/kg body weight) lowered levels of malondialdehyde level, glucose, lipid parameters, AST, ALT, TNF-α and IL-6. The same levels increased the plasma contents of zinc, iron, copper and selenium and activity of antioxidant enzymes (<0.05).
It can be concluded that diabetes decreases TM concentration and antioxidant enzymes and also increases lipid profile, glucose, AST, ALT, TNF-α and IL-6 concentrations. Inclusion of SPM supplementing (20 and 30 mg/kg body weight) increased some TMs and antioxidant enzymes. SPM may provide TMs for synthesis of antioxidant enzymes which subsequently reduce lipid profile, glucose concentration and anti-inflammatory responses.
脂质过氧化和高血糖是糖尿病的常见症状。微藻等天然抗氧化剂可能预防脂质过氧化和高血糖。本研究旨在评估微藻提取物（SPM）对糖尿病大鼠抗氧化和抗炎的作用。
64只大鼠分为八组（每组n = 8），分别口服0、10、20和30mg/kg体重的SPM提取物。实验组包括喂食0mg/kg SPM（DC组）、10mg/kg SPM、20mg/kg SPM和30mg/kg SPM的糖尿病大鼠。健康大鼠分别用0mg/kg SPM（HC组）、10mg/kg SPM、20mg/kg SPM和30mg/kg SPM处理。试验结束时，采集血样，评估血浆中微量元素（TMs）的浓度、生化参数以及肝脏中的抗氧化酶。检测天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。
我们的研究结果表明，糖尿病显著降低了血浆中TMs的浓度和肝脏中的抗氧化酶，同时还增加了丙二醛、葡萄糖、血脂、AST、ALT、TNF-α和IL-6的水平（DC组与HC组相比）。然而，口服SPM（20和30mg/kg体重）可降低丙二醛水平、葡萄糖、血脂参数、AST、ALT、TNF-α和IL-6的水平。同样，这些剂量增加了血浆中锌、铁、铜和硒的含量以及抗氧化酶的活性（P<0.05）。
可以得出结论，糖尿病会降低TMs浓度和抗氧化酶，同时增加血脂、葡萄糖、AST, ALT, TNF-α和IL-6的浓度。补充SPM（20和30mg/kg体重）可增加一些TMs和抗氧化酶。SPM可能为抗氧化酶的合成提供TMs，从而降低血脂、葡萄糖浓度和抗炎反应。
