Pinheiro Liz Helena Moraes, Marañón-Vásquez Guido Artemio, Antunes Leonardo Santos, Proff Peter, Paddenbergb Eva, Kirschneck Christian, Cavalcante-Leão Bianca Lopes, Baratto-Filho Flares, Küchler Erika Calvano, Antunes Lívia Azeredo Alves
Postgraduate Program in Dentistry, School of Dentistry, Fluminense Federal University, Niterói, RJ, Brazil.
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Clin Oral Investig. 2024 Dec 2;28(12):676. doi: 10.1007/s00784-024-06064-9.
To evaluate the association between single nucleotide polymorphisms (SNPs) in cyclooxygenase-2 (COX-2) and external apical root resorption (EARR) after orthodontic treatment.
This study analyzed the orthodontic records of patients from Regensburg University Hospital and two collaborating private practices. EARR was analyzed in the maxillary central incisors and first mandibular molars using pre- and post-treatment cephalometric and panoramic radiographs, respectively. Genomic DNA was obtained from salivary oral cells. Two SNPs in COX-2, rs5275 (3 prime untranslated region variant) and rs689466 (upstream transcript variant, downstream transcript variant), were genotyped using real-time Polymerase chain reaction (PCR). The linear regression models were fitted to assess the effects of the evaluated SNPs at a significance level of 5%.
A total of 143 biologically unrelated patients (mean age: 13.5 ± 4.5 years) were included in the study. The F-test showed that only the regression for EARR in the mandibular molars in the dominant model for rs5275 G allele had a good fit (F = 3.50, P = 0.034). The model showed that individuals carrying at least one G allele for rs5275 had a 17% increase in EARR in the molars compared to common AA homozygotes (exp(B) = 1.17; 95% CI: 1.04, 1.32; P = 0.010). Although an effect of the G allele (rs5275) was detected, this effect did not explain much of the variability in EARR (adjusted R = 0.04).
The results suggested that patients carrying the polymorphic form of the rs5275 were more likely to have EARR in the mandibular molars; therefore, COX-2 could have an effect on EARR.
The knowledge of the genetic background concerning EARR after orthodontic treatment would aid in clinical practice to screen for associated genetic factors and potentially aid its prevention and management, considering that patients' genetic profiles would soon be available.
评估环氧合酶-2（COX-2）单核苷酸多态性（SNP）与正畸治疗后根尖外吸收（EARR）之间的关联。
本研究分析了雷根斯堡大学医院及两家合作私人诊所患者的正畸记录。分别使用治疗前和治疗后的头颅侧位片及全景片对上颌中切牙和下颌第一磨牙的EARR进行分析。从唾液口腔细胞中获取基因组DNA。使用实时聚合酶链反应（PCR）对COX-2基因中的两个SNP，即rs5275（3'非翻译区变体）和rs689466（上游转录变体、下游转录变体）进行基因分型。采用线性回归模型，在5%的显著性水平下评估所评估SNP的效应。
本研究共纳入143名无生物学亲缘关系的患者（平均年龄：13.5±4.5岁）。F检验显示，仅rs5275 G等位基因显性模型中下颌磨牙EARR的回归拟合良好（F = 3.50，P = 0.034）。该模型显示，与常见的AA纯合子相比，携带至少一个rs5275 G等位基因的个体磨牙的EARR增加了17%（exp(B) = 1.17；95%置信区间：1.04，1.32；P = 0.010）。尽管检测到G等位基因（rs5275）的效应，但该效应并不能解释EARR的大部分变异性（调整后R = 0.04）。
结果表明，携带rs5275多态性形式的患者下颌磨牙更易发生EARR；因此，COX-2可能对EARR有影响。
考虑到患者的基因图谱即将可得，了解正畸治疗后EARR的遗传背景知识将有助于临床实践筛查相关遗传因素，并可能有助于其预防和管理。
