Marañón-Vásquez G, Küchler E C, Hermann S, Paddenberg E, Schröder A, Baratto-Filho F, Flores-Mir C, Proff P, Kirschneck C
Department of Pediatric Dentistry, and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Orthodontics, University Medical Centre of Regensburg, Regensburg, Germany.
Eur J Oral Sci. 2023 Apr;131(2):e12916. doi: 10.1111/eos.12916. Epub 2023 Jan 22.
This study evaluated the association between single-nucleotide polymorphisms (SNPs) in vitamin-D-related genes and the amount of external apical root resorption linked to orthodontic treatment. One hundred and forty-three individuals were assessed. The amount of external apical root resorption of upper central incisors (EARR ) and lower first molars (EARR ) were evaluated in radiographs. Seven SNPs were genotyped across four genes including the vitamin D receptor [VDR], group-specific component [GC], cytochrome P450 family 27 subfamily B member 1 [CYP27B1], and cytochrome P450 family 24 subfamily A member 1 [CYP24A1]. Linear regressions were implemented to determine allele-effects on external apical root resorption. Individuals carrying the AA genotype in VDR rs2228570 had a 21% higher EARR than those having AG and GG genotypes (95% CI: 1.03,1.40). EARR in heterozygous rs2228570, was 12% lower than for homozygotes (95%CI: 0.78,0.99). Participants with the CCG haplotype (rs1544410-rs7975232-rs731236) in VDR had an EARR 16% lower than those who did not carry this haplotype. Regarding CYP27B1 rs4646536, EARR in participants who had at least one G allele was 42% lower than for homozygotes AA (95%CI: 0.37,0.93). Although these results did not remain significant after multiple testing adjustment, potential associations may still be suggested. Further replication studies are needed to confirm or refute these findings.
本研究评估了维生素D相关基因中的单核苷酸多态性（SNP）与正畸治疗相关的根尖外吸收量之间的关联。对143名个体进行了评估。在X线片上评估上颌中切牙的根尖外吸收量（EARR）和下颌第一磨牙的根尖外吸收量（EARR）。对包括维生素D受体[VDR]、组特异性成分[GC]、细胞色素P450家族27亚家族B成员1[CYP27B1]和细胞色素P450家族24亚家族A成员1[CYP24A1]在内的四个基因中的7个SNP进行了基因分型。采用线性回归来确定等位基因对根尖外吸收的影响。VDR rs2228570位点携带AA基因型的个体的EARR比携带AG和GG基因型的个体高21%（95%CI：1.03，1.40）。rs2228570杂合子的EARR比纯合子低12%（95%CI：0.78，0.99）。VDR中具有CCG单倍型（rs1544410-rs7975232-rs731236）的参与者的EARR比未携带该单倍型的参与者低16%。关于CYP27B1 rs4646536，至少有一个G等位基因的参与者的EARR比纯合子AA低42%（95%CI：0.37，0.93）。尽管在多重检验校正后这些结果不再显著，但仍可能提示潜在的关联。需要进一步的重复研究来证实或反驳这些发现。
