Sharab L Y, Morford L A, Dempsey J, Falcão-Alencar G, Mason A, Jacobson E, Kluemper G T, Macri J V, Hartsfield J K
Department of Oral Health Practice, University of Kentucky College of Dentistry, Lexington, KY, USA.
Department of Oral Health Science, University of Kentucky College of Dentistry, Lexington, KY, USA.
Orthod Craniofac Res. 2015 Apr;18 Suppl 1(Suppl 1):71-82. doi: 10.1111/ocr.12078.
As genetic variation accounts for two-thirds of the variation in external apical root resorption (EARR) concurrent with orthodontic treatment, we analyzed the association of selected genetic and treatment-related factors with EARR concurrent with orthodontic treatment.
This case-control study of 134 unrelated, orthodontically treated Caucasian individuals was conducted in part at an Indiana Private Practice, Indiana University and the University of Kentucky.
Utilizing a research data bank containing information from ~1450 orthodontically treated patients, pre- and post-treatment radiographs from 460 individuals were evaluated for EARR of the four permanent maxillary incisors. Sixty-seven unrelated Caucasians with moderate to severe EARR were identified and were age-/sex-matched with orthodontically treated Caucasian controls yielding 38 females and 29 males per group. Factors tested for an association with EARR included the following: 1) treatment duration, 2) extraction of maxillary premolars, 3) numerous cephalometric measurements, and 4) DNA polymorphisms within/near candidate genes in a pathway previously implicated in EARR such as the purinergic-receptor-P2X, ligand-gated ion channel 7 (P2RX7; rs208294, rs1718119, and rs2230912), caspase-1 (CASP1; rs530537, rs580253, and rs554344), interleukin-1 beta (IL1B; rs1143634), interleukin-1 alpha (IL1A; rs1800587), and interleukin-1 receptor antagonist (IL1RA; rs419598) genes. Stepwise logistic regression was utilized to identify the factors significantly associated (significance taken at or less than the layered Bonferroni correction alpha) with the occurrence of EARR.
A long length of treatment and the presence of specific genotypes for P2RX7 SNP rs208294 were significantly associated with EARR.
EARR occurrence was associated with both genetic and treatment-related variables, which together explained 25% of the total variation associated with EARR in the sample tested.
由于基因变异占正畸治疗同期根尖外吸收（EARR）变异的三分之二，我们分析了所选基因因素和治疗相关因素与正畸治疗同期EARR的关联。
这项病例对照研究纳入了134名无亲缘关系、接受正畸治疗的白种人个体，部分研究在印第安纳州的一家私人诊所、印第安纳大学和肯塔基大学开展。
利用一个包含约1450名接受正畸治疗患者信息的研究数据库，对460名个体治疗前和治疗后的X光片进行评估，以确定上颌四颗恒牙的EARR情况。识别出67名患有中度至重度EARR的无亲缘关系白种人，并将其与接受正畸治疗的白种人对照进行年龄/性别匹配，每组有38名女性和29名男性。检测与EARR相关的因素包括：1）治疗持续时间；2）上颌前磨牙拔除情况；3）多项头影测量指标；4）先前涉及EARR的通路中候选基因内部或附近的DNA多态性，如嘌呤能受体P2X、配体门控离子通道7（P2RX7；rs208294、rs1718119和rs2230912）、半胱天冬酶-1（CASP1；rs530537、rs580253和rs554344）、白细胞介素-1β（IL1B；rs1143634）、白细胞介素-1α（IL1A；rs1800587）和白细胞介素-1受体拮抗剂（IL1RA；rs419598）基因。采用逐步逻辑回归来识别与EARR发生显著相关的因素（显著性水平设定为小于或等于分层Bonferroni校正α）。
治疗时间长以及P2RX7 SNP rs208294的特定基因型与EARR显著相关。
EARR的发生与基因和治疗相关变量均有关联，二者共同解释了所检测样本中与EARR相关的总变异的25%。
