Li Tongli, Feng Jintang, Ding Wenyu, Zhang Zhang
Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin 300052, China.
Department of Radiology, Tianjin Chest Hospital, Tianjin 300222, China.
ACS Omega. 2024 Nov 11;9(47):47145-47155. doi: 10.1021/acsomega.4c07573. eCollection 2024 Nov 26.
Myocardial infarction (MI) is characterized by rapid onset and high mortality. Due to the complexity of its pathological process, targeted therapy for MI has been a long-standing challenge. Macrophages, as immune cells, selectively accumulate in the infarct area after MI. This inherent targeting ability, coupled with excellent immune evasion capabilities, makes macrophages an ideal source for targeted delivery to damaged myocardium as a biomimetic delivery carrier. Therefore, in this work, we aimed to develop biomimetic nanoparticles for specific targeted drug delivery to efficiently treat MI. Using cell membrane-coated nanotechnology, we prepared resveratrol-loaded nanoparticles (RESNPs) using poly(lactic--glycolic acid) (PLGA) nanoparticles, and then coated the surface of RESNPs with macrophage membranes to prepare biomimetic nanoparticles MM/RESNPs. We then described the physical properties of MM/RESNPs, and their targeting, therapeutic effects, and safety were evaluated in a MI mouse model. The results show that MM/RESNPs exhibited good biocompatibility and could effectively target damaged myocardial sites. MM/RESNPs were found to considerably improve cardiac function and reduce the infarct size in MI mice. Furthermore, MM/RESNPs demonstrated good biosafety after long-term administration. Macrophage membrane-coated biomimetic nanoparticles (MM/RESNPs) exhibit excellent nanoperformance and biosafety, enabling targeted drug delivery and achieving safe and effective treatment for MI.
心肌梗死（MI）具有发病迅速和死亡率高的特点。由于其病理过程复杂，针对MI的靶向治疗一直是一项长期挑战。巨噬细胞作为免疫细胞，在MI后会选择性地聚集在梗死区域。这种固有的靶向能力，再加上出色的免疫逃逸能力，使巨噬细胞成为作为仿生递送载体靶向递送至受损心肌的理想来源。因此，在本研究中，我们旨在开发用于特异性靶向药物递送的仿生纳米颗粒，以有效治疗MI。利用细胞膜包被纳米技术，我们使用聚乳酸-乙醇酸共聚物（PLGA）纳米颗粒制备了负载白藜芦醇的纳米颗粒（RESNPs），然后用巨噬细胞膜包被RESNPs的表面，制备了仿生纳米颗粒MM/RESNPs。然后我们描述了MM/RESNPs的物理性质，并在MI小鼠模型中评估了它们的靶向性、治疗效果和安全性。结果表明，MM/RESNPs表现出良好的生物相容性，能够有效地靶向受损心肌部位。发现MM/RESNPs可显著改善MI小鼠的心脏功能并减小梗死面积。此外，长期给药后MM/RESNPs显示出良好的生物安全性。巨噬细胞膜包被的仿生纳米颗粒（MM/RESNPs）表现出优异的纳米性能和生物安全性，能够实现靶向药物递送并对MI进行安全有效的治疗。
