微小RNA-103表达与2型糖尿病的关联
Association of MicroRNA-103 expression with type 2 diabetes mellitus.
作者信息
Rafiee Mahnoosh, Sadeghi Farzin, Mirzapour Ali, Khafri Soraya, Korani Bahare, Al-E-Ahmad Atiyeh, Parsian Hadi
机构信息
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
出版信息
Caspian J Intern Med. 2024 Oct 19;16(1):106-113. doi: 10.22088/cjim.16.1.106. eCollection 2025 Winter.
BACKGROUND
Type 2 diabetes mellitus (T2DM), the most prevalent metabolic disorder worldwide, has genetic, epigenetic, and environmental contributors. Among them, circulating microRNAs (miRNAs/miRs) have attracted considerable attention. This study aimed to investigate the regulatory role of miR-103 in T2DM development.
METHODS
The miR-103 expression was evaluated in 33 participants with T2DM compared with 38 healthy controls using quantitative real-time polymerase chain reaction (q-RT PCR). Fasting blood glucose (FBS), fasting insulin (Fin), hemoglobin A1c (HbA1c), triglycerides (TG), and cholesterol (Chol) were also determined in serum. Basal insulin resistance and sensitivity were determined using the homeostasis model assessment insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI), respectively.
RESULTS
There was a significant decrease in miR-103 levels in patients with T2DM (p<0.002). Serum miR-103 expression levels showed a strong correlation with age, FBS, HbA1c, TG, Chol levels, and HOMA-IR and QUICKI indices.
CONCLUSION
The downregulation of miR-103 in T2DM patients suggests the diagnostic potential of miR-103 evaluation in conjunction with other diagnostic approaches.
背景
2型糖尿病（T2DM）是全球最常见的代谢紊乱疾病，有遗传、表观遗传和环境等多种致病因素。其中，循环微小RNA（miRNA/miR）已引起广泛关注。本研究旨在探讨miR-103在T2DM发生发展中的调控作用。
方法
采用定量实时聚合酶链反应（q-RT PCR）对33例T2DM患者和38例健康对照者的miR-103表达进行评估。同时测定血清中的空腹血糖（FBS）、空腹胰岛素（Fin）、糖化血红蛋白（HbA1c）、甘油三酯（TG）和胆固醇（Chol）。分别采用稳态模型评估胰岛素抵抗（HOMA-IR）和定量胰岛素敏感性检查指数（QUICKI）测定基础胰岛素抵抗和敏感性。
结果
T2DM患者的miR-103水平显著降低（p<0.002）。血清miR-103表达水平与年龄、FBS、HbA1c、TG、Chol水平以及HOMA-IR和QUICKI指数密切相关。
结论
T2DM患者中miR-103的下调提示miR-103评估与其他诊断方法联合应用具有诊断潜力。
Zotero 插件