Lou Ning, Meng Xiangui, Yu Tiexi, Li Weiquan, Lv Xin, Han Weiwei, Xiao Wen, Shi Ying
Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
Pharmgenomics Pers Med. 2024 Nov 26;17:521-534. doi: 10.2147/PGPM.S469247. eCollection 2024.
Studies have found that RNA-binding proteins (RBPs) are participated in the occurrence or development of tumours. However, the role of processing of precursor family (POP family) in clear cell renal cell carcinoma (ccRCC) has not been studied yet. Here, we analyzed the expression and prognostic value of POP family in ccRCC analyzed and subsequently revealed the relationship between POP7 and immune infiltration in ccRCC patients.
POP family expression in cancer and normal tissues was analyzed in Cancer Genome Atlas pan-cancer (TCGA-pan-cancer). Kaplan-Meier (KM) survival analysis, univariable and multivariable analysis demonstrated the survival of ccRCC with POP family in Kidney Clear Cell Carcinoma (TCGA-KIRC). POP7 mRNA and protein expression were verified by Gene Expression Omnibus (GEO) data, the quantitative real-time polymerase chain reaction (qRT-PCR), and Office of Cancer Clinical Proteomics Research (CPTAC). The diagnostic ability of POP7 mRNA and protein expression was achieved with ROC curves. Gene Set Enrichment Analysis (GSEA) and TiMER2 evaluated pathogenesis role and immune infiltration of POP7in ccRCC.
There is a significant difference in expression of POP family in TCGA-pan-cancer, especially in ccRCC. KM survival analysis, univariable and multivariable analysis demonstrated low expression of POP7 and was associated with poor OS and poor DFS. GEO data, the qRT-PCR, and CPTAC verified the high expression of POP7 mRNA and protein in ccRCC. ROC curves verified a valuable diagnostic ability of POP7 in mRNA and protein expression. GSEA demonstrated POP7 was associated with CD8+cells, CD4+cells, natural killer (NK) cells, and helper T (TH1) cells. TiMER2 results indicated POP7 had a positive correlation with T cell regulatory (Tregs) and myeloid-derived suppressor cells (MDSC) in ccRCC and was an immunosuppressor for ccRCC.
POP7 was a reliable immunosuppressor, predictor and biomarker for ccRCC.
研究发现RNA结合蛋白（RBPs）参与肿瘤的发生或发展。然而，前体家族加工蛋白（POP家族）在肾透明细胞癌（ccRCC）中的作用尚未得到研究。在此，我们分析了POP家族在ccRCC中的表达及预后价值，并随后揭示了POP7与ccRCC患者免疫浸润之间的关系。
在癌症基因组图谱泛癌（TCGA-泛癌）中分析了POP家族在癌组织和正常组织中的表达。Kaplan-Meier（KM）生存分析、单变量和多变量分析证明了POP家族在肾透明细胞癌（TCGA-KIRC）中对ccRCC生存的影响。通过基因表达综合数据库（GEO）数据、定量实时聚合酶链反应（qRT-PCR）和癌症临床蛋白质组学研究办公室（CPTAC）验证了POP7 mRNA和蛋白的表达。通过ROC曲线评估POP7 mRNA和蛋白表达的诊断能力。基因集富集分析（GSEA）和TiMER2评估了POP7在ccRCC中的发病机制作用和免疫浸润情况。
POP家族在TCGA-泛癌中的表达存在显著差异，尤其是在ccRCC中。KM生存分析、单变量和多变量分析表明POP7低表达与较差的总生存期（OS）和无病生存期（DFS）相关。GEO数据、qRT-PCR和CPTAC验证了ccRCC中POP7 mRNA和蛋白的高表达。ROC曲线验证了POP7在mRNA和蛋白表达方面具有有价值的诊断能力。GSEA表明POP7与CD8+细胞、CD4+细胞、自然杀伤（NK）细胞和辅助性T（TH1）细胞相关。TiMER2结果表明，在ccRCC中POP7与调节性T细胞（Tregs）和髓源性抑制细胞（MDSC）呈正相关，是ccRCC的免疫抑制因子。
POP7是ccRCC可靠的免疫抑制因子、预测指标和生物标志物。
