Swain Bharati, Gupta Prateek, Yadav Deepanker
Department of Botany, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, 495009, India.
Department of Biological Sciences, SRM University AP, Neerukonda, Andhra Pradesh, 522240, India.
Biochem Genet. 2024 Dec 2. doi: 10.1007/s10528-024-10979-z.
Annexins are a ubiquitous, evolutionarily conserved group of Ca-dependent phospholipid-binding proteins. They are a family of less numerous and more varied proteins that form a unique monophyletic group. They play an important role in various abiotic and biotic stress responses through Ca-mediated signaling. Chickpea (Cicer arietinum L.) is one of the most widely grown legume crops in the world. In recent years, intensive research has been carried out to identify and elucidate genes and molecular pathways that control stress responses in plants. The availability of the chickpea genome has hastened the functional genomics of chickpea. In the current study, we attempted Genome-wide identification and in silico analysis of Annexins in chickpea. Thirteen annexin sequences have been identified in the chickpea genome. Four conserved annexin domains were found in ten annexin members, while three annexins CaAnn5, CaAnn12, and CaAnn13, showed three, two, and one conserved domain, respectively. The gene structure analysis showed the presence of multiple exons in all thirteen annexins. Most Annexin genes are composed of 3-5 introns. Their chromosomal locations showed that out of thirteen genes, ten could be mapped on four chromosomes. Three genes were placed on the scaffold regions. The promoter sequence analysis of all thirteen annexins showed the presence of various elements related to growth and development and response to different phytohormones and abiotic stress. The gene expression data of different annexins in various tissues like leaf, shoot, root, flower bud, and young pod showed their differential expression. Analysis of expression data of roots in drought stress showed their differential expression with the different stages of plant growth. Overall, the current findings show the possible role of CaAnns in different stages of plant growth and development in normal and stressful conditions. Moreover, these findings will be helpful in the further characterization of CaAnn genes and their promoters.
膜联蛋白是一类普遍存在且在进化上保守的钙依赖性磷脂结合蛋白。它们是一组数量较少且种类更多样的蛋白质家族，构成一个独特的单系群。它们通过钙介导的信号传导在各种非生物和生物胁迫反应中发挥重要作用。鹰嘴豆（Cicer arietinum L.）是世界上种植最广泛的豆类作物之一。近年来，人们开展了深入研究以鉴定和阐明控制植物胁迫反应的基因和分子途径。鹰嘴豆基因组的可得性加速了鹰嘴豆的功能基因组学研究。在本研究中，我们尝试对鹰嘴豆中的膜联蛋白进行全基因组鉴定和电子分析。在鹰嘴豆基因组中鉴定出了13个膜联蛋白序列。在10个膜联蛋白成员中发现了4个保守的膜联蛋白结构域，而3个膜联蛋白CaAnn5、CaAnn12和CaAnn13分别显示出3个、2个和1个保守结构域。基因结构分析表明，所有13个膜联蛋白中都存在多个外显子。大多数膜联蛋白基因由3 - 5个内含子组成。它们的染色体定位表明，在13个基因中，有10个可以定位到4条染色体上。3个基因位于支架区域。对所有13个膜联蛋白的启动子序列分析表明，存在与生长发育以及对不同植物激素和非生物胁迫反应相关的各种元件。不同膜联蛋白在叶片、茎、根、花芽和幼荚等不同组织中的基因表达数据显示了它们的差异表达。干旱胁迫下根的表达数据分析表明，它们在植物生长的不同阶段存在差异表达。总体而言，当前的研究结果表明CaAnns在正常和胁迫条件下植物生长发育的不同阶段可能发挥的作用。此外，这些发现将有助于进一步表征CaAnn基因及其启动子。
