Lowe David, Davidson Whitni, Wilkins Kimberly, Baird Nicolle
National Center for Immunizations and Respiratory Diseases, Coronavirus and Other Respiratory Viruses Laboratory Branch, U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
National Center for Emerging and Zoonotic Infectious Diseases, Poxvirus and Rabies Branch, U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
Methods Mol Biol. 2025;2860:15-26. doi: 10.1007/978-1-0716-4160-6_2.
During the 2022 and ongoing mpox outbreak, PCR diagnostics were already within the Laboratory Response Network (LRN) laboratories and additionally rapidly deployed to detect monkeypox (mpox) virus in patients and aid in accurate diagnosis. This accuracy is dependent on proper collection, shipment, and adherence to PCR procedure. This book chapter discusses effective collection, storage, and transportation for a real-time PCR to detect monkeypox virus nucleic acid.
在2022年及仍在持续的猴痘疫情期间，聚合酶链反应（PCR）诊断检测已在实验室反应网络（LRN）实验室开展，此外还迅速部署用于检测患者体内的猴痘病毒，以协助进行准确诊断。这种准确性取决于正确的样本采集、运输以及对PCR程序的严格遵守。本章将讨论用于检测猴痘病毒核酸的实时PCR的有效样本采集、储存和运输方法。
