Verbakel Lina, Lenaerts Cynthia, Vranken Jerom, Marchal Elisabeth, Vanden Broeck Jozef
Molecular Developmental Physiology and Signal Transduction, Biology Department, KU Leuven, Naamsestraat 59 Box 2465, B-3000 Leuven, Belgium; Nagi Bioscience SA, EPFL Innovation Park (M), Rue des Jordils 1A 1025 Saint-Sulpice, Switzerland.
Molecular Developmental Physiology and Signal Transduction, Biology Department, KU Leuven, Naamsestraat 59 Box 2465, B-3000 Leuven, Belgium.
J Insect Physiol. 2025 Mar;161:104736. doi: 10.1016/j.jinsphys.2024.104736. Epub 2024 Nov 30.
The process of molting represents a critical phase in the life cycle of arthropods, marking periods of growth and development. Central to this process is the eclosion hormone (EH), a neurohormone that plays a pivotal role in initiating and regulating the complex sequence of events leading to successful molting in holometabolan species. Very little information is available in Hemimetabola, which display a different kind of development characterized by gradual changes. This paper reports on the identification of the two EH precursors and the EH receptor (EHR), a guanylyl cyclase, in a hemimetabolan pest species, the desert locust, Schistocerca gregaria. Using qRT-PCR, an in-depth profiling study of Schgr-EH-1, -2 and Schgr-EHR transcripts was performed. Silencing of Schgr-EH-1, -2 and Schgr-EHR resulted in lethality at the expected time of ecdysis, thereby showing their crucial role during this process.
蜕皮过程是节肢动物生命周期中的一个关键阶段，标志着生长和发育时期。这一过程的核心是羽化激素（EH），一种神经激素，在全变态类物种中，它在启动和调节导致成功蜕皮的复杂事件序列中起着关键作用。关于半变态类昆虫的信息非常少，它们表现出一种以渐进变化为特征的不同发育方式。本文报道了在一种半变态类害虫——沙漠蝗虫（Schistocerca gregaria）中鉴定出两种EH前体和EH受体（EHR，一种鸟苷酸环化酶）。使用qRT-PCR对Schgr-EH-1、-2和Schgr-EHR转录本进行了深入的表达谱研究。沉默Schgr-EH-1、-2和Schgr-EHR会导致在预期的蜕皮时间出现致死现象，从而表明它们在此过程中的关键作用。
