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拉丁美洲各地区集市和市场上整只鱿鱼和枪乌贼的分子鉴定。

Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America.

作者信息

Paiva Bianca Lima, Rodrigues Alan Erik Souza, Almeida Igor Oliveira de Freitas, Silva Kamila de Fatima, Haimovici Manuel, Markaida Unai, Charvet Patricia, Faria Vicente Vieira, Batista Bruno B, Tomás Acácio Ribeiro Gomes, Rodrigues-Filho Luis Fernando da Silva, Ready Jonathan Stuart, Sales João Bráullio de Luna

机构信息

Grupo de Investigação Biológica Integrada, Centro de Estudos Avançados da Biodiversidade, Universidade Federal do Pará, Av. Perimetral 01, PCT-Guamá, Terreno 11, Belém, PA, CEP: 66075-750, Brazil.

Laboratório de Recursos Demersais e Cefalópodes, Universidade Federal do Rio Grande (FURG), Caixa Postal 474, Rio Grande, RS, CEP 96201-900, Brazil.

出版信息

Sci Rep. 2024 Dec 3;14(1):30007. doi: 10.1038/s41598-024-81134-2.

Abstract

The commercial importance of cephalopods has increased considerably, being an important fishing resource. However, during the preparation for commercialization of those species, they suffer the process known as "finning" which includes removing and separating the head, arm, skin or even having the body structure cut into rings, which ends up making it difficult or often prevents the identification of the species, which can lead to replacements. In this sense, the present study aimed to use the large ribosomal region, rrnL (16S rDNA) to genetically identify cephalopod species sold in markets and fairs in Latin America. Whole and processed samples were collected from supermarkets and directly from local fishers the approximate collection location. Each generated sequence was submitted to the blastn portal for molecular comparison and included in the database for subsequent genetic identification. Our results indicate labeling errors in samples from the State of Pará that contained the species Dosidicus gigas found only in the Pacific Ocean and were generically labeled as "National Lula". No type of substitution was found among the samples that were being sold at fairs and markets, only labeling errors. Thus, our results demonstrate the effectiveness of the rrnL for identifying species and evaluating labeling errors.

摘要

头足类动物的商业重要性已大幅提高,成为一种重要的渔业资源。然而,在这些物种商业化准备过程中,它们会经历所谓的“割鳍”过程,这包括把头、腕、皮去掉或分离,甚至将身体结构切成环状,这最终使得物种鉴定变得困难,甚至常常无法鉴定,进而可能导致物种被替换。从这个意义上说,本研究旨在利用大核糖体区域rrnL(16S rDNA)对拉丁美洲市场和集市上销售的头足类物种进行基因鉴定。从超市以及直接从当地渔民处(大致采集地点)收集了完整和经过加工的样本。每个生成的序列都提交到blastn平台进行分子比对,并纳入数据库以便后续进行基因鉴定。我们的结果表明,帕拉州样本中存在标签错误,这些样本包含仅在太平洋发现的茎柔鱼,却被一概标记为“国产鱿鱼”。在集市和市场上销售的样本中未发现任何种类的替换情况,只有标签错误。因此,我们的结果证明了rrnL在鉴定物种和评估标签错误方面的有效性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/846a/11612498/d8a3cf70f1d6/41598_2024_81134_Fig1_HTML.jpg

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