Xu Qingqing, Chen Yuxin, Ni Xinyan, Zhuang Hanying, Cao Shenxi, Zhao Liwei, Wang Leying, Chen Jianhui, Yang Wen Z, Zeng Wenwen, Li Xi, Sun Hongbin, Shen Wei L
Biology Science Institutes, Chongqing Medical University, Chongqing 400016, China.
School of Life Science and Technology & Shanghai Clinical Research and Trial Center, ShanghaiTech University, Shanghai 201210, China.
Acta Biochim Biophys Sin (Shanghai). 2024 Dec 2;57(7):1139-1150. doi: 10.3724/abbs.2024215.
A better understanding of how sympathetic nerves impact pancreatic function is helpful for understanding diabetes. However, there is still uncertainty and controversy surrounding the roles of sympathetic nerves within the pancreas. To address this, we utilize high-resolution imaging and advanced three-dimensional (3D) reconstruction techniques to study the patterns of sympathetic innervation and morphology in the islets of adult wild-type (WT) and diabetic mice. Our data show that more than ~30% of α/β-cells are innervated by sympathetic nerves in both WT and diabetic mice. Additionally, sympathetic innervated α/β-cells are reduced in diet-induced obese (DIO) mice, whereas sympathetic innervated β-cells are increased in / mice. In addition, chemical pancreatic sympathetic denervation (cPSD) improves glucose tolerance in WT and / mice but decreases glucose tolerance in DIO mice. cPSD also enhances insulin sensitivity in diabetic mice without affecting WT mice. Overall, our findings advance our understanding of diabetes by highlighting the distinctive impact of pancreatic sympathetic innervation on glucose regulation.
更好地理解交感神经如何影响胰腺功能有助于理解糖尿病。然而，围绕交感神经在胰腺中的作用仍存在不确定性和争议。为了解决这个问题，我们利用高分辨率成像和先进的三维（3D）重建技术来研究成年野生型（WT）和糖尿病小鼠胰岛中交感神经支配模式和形态。我们的数据表明，在WT和糖尿病小鼠中，超过约30%的α/β细胞由交感神经支配。此外，在饮食诱导肥胖（DIO）小鼠中，交感神经支配的α/β细胞减少，而在/小鼠中，交感神经支配的β细胞增加。此外，化学性胰腺交感神经去神经支配（cPSD）可改善WT和/小鼠的葡萄糖耐量，但降低DIO小鼠的葡萄糖耐量。cPSD还可增强糖尿病小鼠的胰岛素敏感性，而不影响WT小鼠。总体而言，我们的研究结果通过突出胰腺交感神经支配对葡萄糖调节的独特影响，推进了我们对糖尿病的理解。
