Ghali-Mohammed I, Odetokun I A, Raufu I A, Adetunji V O
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
Iran J Vet Res. 2024;25(2):92-97. doi: 10.22099/IJVR.2024.49643.7307.
is a major cause of poor microbial quality of milk, often resulting from unhygienic milk handling. Milk contamination poses public health concerns. Shiga toxin-producing (STEC) strains in food products, particularly milk, is a critical concern for public health. Limited information exists on the detection of non-O157 pathotypes in milk sold by local processors in Nigeria.
This study aimed to explore the diversity of non-O157 STEC isolates found in commercially available milk in Kwara State, Nigeria, to find the genetic diversity and potential risks associated with these strains.
A subgroup of 18 representative non-O157 STEC isolated from milk samples (n=1225) was selected for whole genome sequencing (WGS) analysis.
Four novel sequence types (ST): ST398, ST540, ST1727, and ST9891 of non-O157 involving five serotypes: O176:H30, O176:H20, O8:H20, O21:H45, and O22:H7, carrying variable proportions of virulence factors, antimicrobial resistance genes, and plasmids, were identified.
This investigation contributes valuable data to the ongoing efforts to ensure food safety and prevent the transmission of strains through dairy products. The findings have implications for public health policies and food quality standards in Kwara State, Nigeria. Improved hygienic practices during milk handling are recommended.
是牛奶微生物质量差的主要原因，通常源于牛奶处理过程不卫生。牛奶污染引发了公共卫生问题。食品产品中，特别是牛奶中的产志贺毒素大肠杆菌（STEC）菌株，是公共卫生的一个关键问题。关于尼日利亚当地加工商销售的牛奶中检测非O157菌型的信息有限。
本研究旨在探索在尼日利亚夸拉州市售牛奶中发现的非O157 STEC分离株的多样性，以找出这些菌株的遗传多样性和潜在风险。
从牛奶样本（n = 1225）中分离出的18株具有代表性的非O157 STEC亚组被选用于全基因组测序（WGS）分析。
鉴定出四种新的序列类型（ST）：非O157的ST398、ST540、ST1727和ST9891，涉及五种血清型：O176:H30、O176:H20、O8:H20、O21:H45和O22:H7，携带不同比例的毒力因子、抗菌抗性基因和质粒。
本调查为正在进行的确保食品安全和防止通过乳制品传播菌株的努力提供了有价值的数据。这些发现对尼日利亚夸拉州的公共卫生政策和食品质量标准有影响。建议在牛奶处理过程中改进卫生操作规范。
