Department of Biological Sciences, College of Basic and Applied Science, Mountain Top University, Kilometre 12, Lagos-Ibadan Expressway, Prayer City, Ogun State, Nigeria.
Department of Biological Sciences, College of Basic and Applied Science, Mountain Top University, Kilometre 12, Lagos-Ibadan Expressway, Prayer City, Ogun State, Nigeria; Department of Food Science, University of Pretoria, Private Bag X 20, Hatfield, 0028 Pretoria, South Africa.
Int J Food Microbiol. 2021 Jun 2;347:109191. doi: 10.1016/j.ijfoodmicro.2021.109191. Epub 2021 Mar 31.
Fresh beef and meat products have been implicated in outbreaks of Shiga toxin-producing Escherichia coli (STEC) worldwide. This study investigated the prevalence of E. coli O157: H7 and non-O157 STEC serogroups in fresh beef in the open market and street vended meat products (n = 180) in Lagos metropolis, Nigeria. A combination of culture media and immunomagnetic separation followed by typing for associated virulence factors and serotypes was performed. Antimicrobial susceptibility testing was performed on the isolated STEC serotypes using the disk diffusion method. A total of 72 STEC serogroup isolates were detected from 61 out of 180 samples. The O157 STEC serotypes were detected in fresh beef, suya, minced meat and tsire with prevalence of 20.8% while non-O157 STEC serogroups were detected in all the samples. Molecular typing revealed 25% (n = 18) of the STEC serogroups showed presence of all the stx1, stx2, eaeA, fliCH7 and rfbEO157 virulence factors while 54.2% (n = 39) possessed a combination of two virulence genes. Multidrug resistance was discovered in 23.6% (n = 17) of the total STEC serogroups. Locally processed ready-to-eat meat products in Lagos metropolis, Nigeria harbour potentially pathogenic multi-drug resistant STEC serogroups that can constitute public health hazard.
新鲜牛肉和肉类产品已被世界范围内的产志贺毒素大肠杆菌（STEC）暴发所牵连。本研究调查了尼日利亚拉各斯大都市开放市场和街头贩卖的新鲜牛肉和肉类产品（n=180）中大肠杆菌 O157：H7 和非 O157 STEC 血清群的流行情况。采用组合培养基和免疫磁分离，然后对相关毒力因子和血清型进行分型。采用纸片扩散法对分离的 STEC 血清型进行了抗生素敏感性测试。从 180 个样本中的 61 个样本中检测到 72 个 STEC 血清群分离株。新鲜牛肉、suya、肉末和 tsire 中检测到 O157 STEC 血清型，流行率为 20.8%，而非 O157 STEC 血清群则存在于所有样本中。分子分型显示，25%（n=18）的 STEC 血清群显示存在所有stx1、stx2、eaeA、fliCH7 和 rfbEO157 毒力因子，而 54.2%（n=39）则存在两种毒力基因的组合。在总 STEC 血清群中发现 23.6%（n=17）具有多药耐药性。尼日利亚拉各斯大都市的本地加工即食肉类产品中存在潜在致病性多药耐药 STEC 血清群，可能构成公共卫生危害。
