Saeed Fiza, Carter Caroline, Kolade John, Brothers Robert Matthew, Liu Hanli
University of Texas at Arlington, Department of Bioengineering, Arlington, Texas, United States.
University of Texas at Arlington, Department of Kinesiology, Arlington, Texas, United States.
J Biomed Opt. 2024 Nov;29(11):117001. doi: 10.1117/1.JBO.29.11.117001. Epub 2024 Nov 29.
Broadband near-infrared spectroscopy (bbNIRS) is useful for the quantification of cerebral metabolism. However, its usefulness has not been explored for broad biomedical applications.
We aimed to quantify the dynamic responses of oxidized cytochrome c oxidase ( ) within the mitochondria to arterial occlusion and the dynamic correlations between hemodynamic ( ) and responses during and after occlusion in forearm tissues.
We recruited 14 healthy participants with two-channel bbNIRS measurements in response to a 5-min forearm arterial occlusion. The bbNIRS system consisted of one shared white-light source and two spectrometers. The modified Beer-Lambert law was applied to determine the occlusion-induced changes in and in the shallow- and deep-tissue layers.
During the 5-min occlusion, dynamic responses in hemodynamics exhibited the expected changes, but remained constant, as observed in the 1- and 3-cm channels. A linear correlation between and was observed only during the recovery phase, with a stronger correlation in deeper tissues. The observation of a constant during the cuff period was consistent with two previous reports. The interpretation of this observation is based on the literature that the oxygen metabolism of the skeletal muscle during arterial occlusion remains unchanged before all oxy-hemoglobin (and oxy-myoglobin) resources are completely depleted. Because a 5-min arterial occlusion is not adequate to exhaust all oxygen supply in the vascular bed of the forearm, the local oxygen supply to the muscle mitochondria maintains redox metabolism uninterrupted by occlusion.
We provide a better understanding of the mitochondrial responses to forearm arterial occlusion and demonstrate the usefulness of bbNIRS.
宽带近红外光谱（bbNIRS）可用于量化脑代谢。然而，其在广泛生物医学应用中的效用尚未得到探索。
我们旨在量化线粒体中氧化型细胞色素c氧化酶（ ）对动脉闭塞的动态反应，以及前臂组织在闭塞期间和之后血流动力学（ ）与 反应之间的动态相关性。
我们招募了14名健康参与者，使用双通道bbNIRS测量来响应5分钟的前臂动脉闭塞。bbNIRS系统由一个共享的白光源和两个光谱仪组成。应用修正的比尔-朗伯定律来确定闭塞引起的浅组织层和深组织层中 和 的变化。
在5分钟的闭塞期间，血流动力学的动态反应呈现出预期的变化，但在1厘米和3厘米通道中观察到 保持恒定。仅在恢复阶段观察到 和 之间存在线性相关性，在更深的组织中相关性更强。在袖带期观察到 恒定与之前的两份报告一致。对这一观察结果的解释基于文献，即动脉闭塞期间骨骼肌的氧代谢在所有氧合血红蛋白（和氧合肌红蛋白）资源完全耗尽之前保持不变。由于5分钟的动脉闭塞不足以耗尽前臂血管床中的所有氧气供应，因此向肌肉线粒体的局部氧气供应维持了不受闭塞干扰的氧化还原代谢。
我们更好地理解了线粒体对前臂动脉闭塞的反应，并证明了bbNIRS的效用。
