Sheep-derived butyrate-producing alleviates diarrhea by shaping the gut microbiota of goat kids.

Newborn goat kids exposed to environmental stress are susceptible to diarrhea due to immature intestinal functions and undeveloped gut microbiota. Butyrate-producing bacteria as next generation probiotics benefit the maintenance of intestinal health, but the mode of regulation is still unclear. Herein, a novel butyrate-producing strain was isolated from sheep rumen and identified as () , thereafter goat kids were treated with to elucidate its regulatory mechanisms on diarrhea. Thirty-six goat kids were assigned to four groups: control (CON), low dose (LCB; supplementation with 5 × 10 CFU/mL of , each with 10 mL/d), middle dose (MCB; supplementation with 5 × 10 CFU/mL of , each with 10 mL/d), high dose (HCB; supplementation with 5 × 10 CFU/mL of , each with 10 mL/d). The experiment lasted for 15 d, and 6 goat kids were randomly selected from each group for slaughter on the last day of the trial. The results showed that this isolate reduced the diarrhea rate ( 0.001) and fecal scores ( 0.001). In the gut, its supplementation inhibited inflammation, increased antioxidant capacity, and regulated intestinal flora. Meanwhile, strengthened the intestinal barrier and altered the jejunum morphology. This evidence suggests that may alleviate diarrhea in goat kids by regulating microbiota, which is directly related to intestinal barrier and immune capacity.