Li Jiahui, Cheng Baoyu, Li Zihe, Cui Yanlei, Yang Haiyan, Liu Weiquan, Zhang Chuanmei, Yu Yongle
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.
College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
Virus Genes. 2025 Feb;61(1):97-109. doi: 10.1007/s11262-024-02125-z. Epub 2024 Dec 3.
Canine parvovirus (CPV) is an important pathogen of dogs and wild carnivores. It is a single-stranded DNA virus with a high mutation frequency and antigenic drift. To research the prevalence and genetic variation of CPV in Shandong, 62 samples from diseased dogs were collected and examined by using PCR for parvovirus. Our results showed that the positivity was 62.9% (n = 39), VP2 gene were sequenced and compared with reference strains. For the parvovirus subtype prevalence, 7 strains were CPV-2a (17.9%) and 32 strains were CPV-2c (82.1%). The results of phylogenetic analysis of VP2 gene of the CPVs showed all 39 isolates formed a major clade and were distantly related to the commercial vaccine strains. By comparing amino acid (aa) sequences, this study discovered new mutations not previously reported which may be related to host range and antigenicity. Moreover, one CPV-2c strain (QN-55) was isolated and cultured on F81 cells, and characterized by whole-genome sequencing. The TCID of this strain was 10/0.1 mL and animal tests have shown that the strain is fatal to infected dogs.
犬细小病毒（CPV）是犬类和野生食肉动物的一种重要病原体。它是一种单链DNA病毒，具有高突变频率和抗原漂移。为研究CPV在山东的流行情况和基因变异，采集了62份患病犬的样本，并用细小病毒PCR进行检测。我们的结果显示阳性率为62.9%（n = 39），对VP2基因进行测序并与参考毒株进行比较。对于细小病毒亚型流行情况，7株为CPV-2a（17.9%），32株为CPV-2c（82.1%）。CPV的VP2基因系统发育分析结果显示，所有39株分离株形成一个主要分支，与商业疫苗株亲缘关系较远。通过比较氨基酸（aa）序列，本研究发现了以前未报道的新突变，这些突变可能与宿主范围和抗原性有关。此外，分离出一株CPV-2c毒株（QN-55）并在F81细胞上进行培养，通过全基因组测序对其进行特征分析。该毒株的半数组织培养感染剂量（TCID）为10/0.1 mL，动物试验表明该毒株对感染犬具有致死性。
