China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Transbound Emerg Dis. 2019 Mar;66(2):897-907. doi: 10.1111/tbed.13100. Epub 2019 Jan 10.
Canine parvovirus 2 (CPV-2) infection is responsible for large numbers of animal deaths worldwide and is one of the most dangerous infectious diseases in young puppies. Twenty-four rectal swabs were collected from dogs with clinical signs of vomiting and haemorrhagic diarrhoea and were initially verified to be infected with CPV-2 using colloidal gold test strips. From the 24 CPV-positive samples, complete genome of 5050-5054 nucleotides was sequenced with a next-generation sequencing platform. Characteristics of the Open Reading Frames from different CPV-2 strains detected in this study were analyzed. Several VP2 point mutations were discovered, and demonstrated the co-circulation of new CPV-2a, new CPV-2b and CPV-2c in Sichuan province of China. The analysis results of the Chinese CPV-2 retrieved from the NCBI nucleotide, showed that new CPV-2a has become the predominant variant in some provinces of China. Phylogenetic analysis of global VP2 and NS1 nucleotide sequences revealed certain correlations among geographical regions, types and circulating time, which lays the foundation for further research concerning the epidemiology, genetic variation, vaccination and molecular evolutionary relationships of the CPV-2 identified at different times and from different regions.
犬细小病毒 2 型（CPV-2）感染是导致全球大量动物死亡的原因之一，也是幼犬最危险的传染病之一。从有呕吐和出血性腹泻临床症状的犬只中采集了 24 份直肠拭子，最初使用胶体金测试条证实这些样本感染了 CPV-2。从 24 份 CPV 阳性样本中，使用下一代测序平台对 5050-5054 个核苷酸的完整基因组进行了测序。分析了本研究中检测到的不同 CPV-2 株的开放阅读框特征。发现了几个 VP2 点突变，并证实了新 CPV-2a、新 CPV-2b 和 CPV-2c 在我国四川省的共同流行。从 NCBI 核苷酸检索到的中国 CPV-2 的分析结果表明，新 CPV-2a 已成为中国一些省份的主要变异株。全球 VP2 和 NS1 核苷酸序列的系统进化分析表明，地理区域、类型和流行时间之间存在一定的相关性，这为进一步研究不同时间和不同地区分离的 CPV-2 的流行病学、遗传变异、疫苗接种和分子进化关系奠定了基础。
