Chen Yiqian, Cheng Lixiang, Guan Xiaoying, Liang Yi, Xue Yanjiao, Zhao Wenyan, Zhang Ziyue, Chang Xiaoyan, Liang Liqin, Gao Gang
College of Life Science, Shanxi Normal University, Taiyuan, China.
College of Life Science, Shanxi Normal University, Taiyuan, China.
Biochem Biophys Res Commun. 2025 Jan;742:151105. doi: 10.1016/j.bbrc.2024.151105. Epub 2024 Nov 30.
Cysteine-rich polycomb-like proteins (CPP) are crucial in regulating plant stress responses while the underlying functions of CPP involving plant- Ralstonia solanacaearum interaction remain unknown. Here, we showed the expression patterns of a potato CPP gene (StCPP3) under phytohormone treatments, biotic and abiotic stressed and its role in resistance against of R. solanacaearum infection by loss- and gain-of-function approaches. StCPP3 expression were up-regulated with methyl jasmonate (MeJA) and abscisic acid (ABA) while down-regulated under salicylic acid (SA), brassinosteroids (BR), high salt or low temperature treatment. Silencing the homolog gene (NbCPP3) in Nicotiana benthamiana enhanced resistance to R. solanacaearum. Over-expressing StCPP3 in Arabidopsis increased susceptibility and decreased activity of some defense-related enzymes, suggesting its role in suppressing hypersensitive cell death and reducing PR1 gene expression. In addition, we found that StCPP3 could interact with Type III secretion protein HrpB7 from R. solanacaearum. These results provide new insight into the mechanism of CPP's involvement in plant-pathogen interactions.
富含半胱氨酸的多梳样蛋白（CPP）在调节植物应激反应中起关键作用，而CPP在植物与青枯雷尔氏菌相互作用中的潜在功能尚不清楚。在此，我们通过功能缺失和功能获得方法展示了马铃薯CPP基因（StCPP3）在植物激素处理、生物和非生物胁迫下的表达模式及其在抵抗青枯雷尔氏菌感染中的作用。StCPP3的表达在茉莉酸甲酯（MeJA）和脱落酸（ABA）处理下上调，而在水杨酸（SA）、油菜素内酯（BR）、高盐或低温处理下下调。沉默本氏烟草中的同源基因（NbCPP3）增强了对青枯雷尔氏菌的抗性。在拟南芥中过表达StCPP3增加了易感性并降低了一些防御相关酶的活性，表明其在抑制过敏细胞死亡和降低PR1基因表达中的作用。此外，我们发现StCPP3可以与青枯雷尔氏菌的III型分泌蛋白HrpB7相互作用。这些结果为CPP参与植物-病原体相互作用的机制提供了新的见解。
