Jiang Le, Zhu Min, Huang Ying, Zhang Quanyan
Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi 276000, China.
College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, China.
Int J Mol Sci. 2025 Jun 10;26(12):5528. doi: 10.3390/ijms26125528.
Cysteine-rich polycomb-like protein (CPP) transcription factors play critical roles in plant growth, development, and responses to stresses and hormone signaling. However, the research on the gene family remains unexplored in apple. In this study, a total of 10 genes (-) were identified and unevenly distributed across seven scaffolds. Phylogenetic and conserved motif analyses revealed that these 10 CXC domain-containing MdCPPs could be classified into three subfamilies. Evolutionary tree and synteny analyses demonstrated that apple shared the highest number of orthologous gene pairs with white pear compared to . By analyzing the gene promoter, a large number of -acting elements related to hormone and stress response were discovered. In addition, transcriptomic data demonstrated tissue-specific expression patterns of genes, with and showing the highest expression in buds and leaves. The qRT-PCR results indicated that genes have different expression responses to SA, GA, JA, and IAA treatments. Notably, , , , and were significantly upregulated under different hormone treatments. Among them, the upregulation of was the most significant. These findings establish a foundation for further functional characterization of and provide theoretical support for their potential applications in apple genetic improvement and agricultural production.
富含半胱氨酸的多梳样蛋白（CPP）转录因子在植物生长、发育以及对胁迫和激素信号的响应中发挥着关键作用。然而，苹果中该基因家族的研究仍未开展。本研究共鉴定出10个基因（-），它们不均匀地分布在7个支架上。系统发育和保守基序分析表明，这10个含CXC结构域的MdCPPs可分为三个亚家族。进化树和共线性分析表明，与相比，苹果与白梨共享的直系同源基因对数量最多。通过分析基因启动子，发现了大量与激素和胁迫响应相关的顺式作用元件。此外，转录组数据显示了基因的组织特异性表达模式，其中和在芽和叶中表达最高。qRT-PCR结果表明，基因对SA、GA、JA和IAA处理有不同的表达响应。值得注意的是，、、、和在不同激素处理下显著上调。其中，的上调最为显著。这些发现为进一步对进行功能表征奠定了基础，并为其在苹果遗传改良和农业生产中的潜在应用提供了理论支持。
