Kudo Kei, Nishimura Takehiro, Miyako Kei, Suenaga Hikaru, Shin-Ya Kazuo
Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, 135-0064, Japan.
Technology Research Association for Next Generation Natural Products Chemistry, Tokyo, 135-0064, Japan.
J Antibiot (Tokyo). 2025 Jan;78(2):126-130. doi: 10.1038/s41429-024-00793-5. Epub 2024 Dec 3.
A new derivative of cannabigerolic acid, designated as iso-cannabigerolic acid (iso-CBGA), a member of the cannabinoid family, and its precursor iso-olivetolic acid (iso-OA) were discovered from the culture of the engineered Streptomyces avermitilis heterologously expressing the genes responsible for cannabigerolic acid biosynthesis. Structural determination revealed an iso-pentyl moiety, which may arise from the biosynthetic precursor pool present in S. avermitilis. We describe herein the culture, isolation, structure determination and antibacterial activities of iso-CBGA and iso-OA.
从经基因工程改造、异源表达负责大麻二酚酸生物合成相关基因的阿维链霉菌培养物中，发现了大麻二酚酸的一种新衍生物，命名为异大麻二酚酸（iso-CBGA），它是大麻素家族的一员，以及其前体异橄榄酸（iso-OA）。结构测定显示存在一个异戊基部分，这可能源于阿维链霉菌中存在的生物合成前体库。本文我们描述了异大麻二酚酸和异橄榄酸的培养、分离、结构测定及抗菌活性。
