Yang Lingwei, Fang Junchao, Wang Jingxin, Hui Suozhen, Zhou Liang, Xu Bo, Chen Yujuan, Zhang Yuanyuan, Lai Changkai, Jiao Guiai, Sheng Zhonghua, Wei Xiangjin, Shao Gaoneng, Xie Lihong, Wang Ling, Chen Ying, Zhao Fengli, Hu Shikai, Hu Peisong, Tang Shaoqing
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.
Front Plant Sci. 2023 Jul 24;14:1222288. doi: 10.3389/fpls.2023.1222288. eCollection 2023.
3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in has not been reported yet. In this study, we identified 22 genes in rice, which are unevenly distributed on nine chromosomes. The gene family is divided into six subclasses. Many -acting elements related to plant growth, light, hormone, and stress response were enriched in the promoters of genes. Gene duplication played a crucial role in the expansion of the gene family and underwent a strong purifying selection. Quantitative Real-time polymerase chain reaction (qRT-PCR) results revealed that most genes are constitutively expressed. We also revealed that genes responded differently to exogenous cadmium stress in and background, and the genes with higher expression in leaves and seeds may have functions under cadmium stress. This study provides a basis for further understanding the functions of genes and the biosynthesis of VLCFA in rice.
3-酮脂酰辅酶A合酶（KCS）是植物中极长链脂肪酸（VLCFA）合成的关键限速酶，它决定了VLCFA的碳链长度。然而，尚未有对[具体物种]中KCSs的全面研究报道。在本研究中，我们在水稻中鉴定出22个[基因名称]基因，它们在九条染色体上分布不均。[基因名称]基因家族分为六个亚类。许多与植物生长、光、激素和应激反应相关的顺式作用元件在[基因名称]基因的启动子中富集。基因复制在[基因名称]基因家族的扩张中起关键作用，并经历了强烈的纯化选择。实时定量聚合酶链反应（qRT-PCR）结果显示，大多数[基因名称]基因组成型表达。我们还发现，[基因名称]基因在[不同背景]对外源镉胁迫的反应不同，在叶片和种子中高表达的[基因名称]基因可能在镉胁迫下具有功能。本研究为进一步了解[基因名称]基因的功能以及水稻中VLCFA的生物合成提供了依据。
