Tope-Fatukasi Blessing P, Afolabi Olakunle B, Ogunnusi Tolutope A, Akpor Oghenerobor B
Department of Biological Sciences, College of Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Department of Chemical Sciences, College of Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Int Microbiol. 2024 Dec 4. doi: 10.1007/s10123-024-00620-9.
Probiotics have been shown to improve host access to vitamins, generate vitamins and improve gastrointestinal tract functions throughout transit yeasts which are indicated to potentially function as probiotics with some health benefits. The aim of this study was to evaluate the probiotic potential of yeast isolates from banana and plantain peels. Yeast strains were isolated from ripe banana and plantain peels, using the standard procedures. Isolates that showed negativity for pathogenicity assays were used for in vitro and in vivo probiotic screening. For the in vivo assay, 30 male Wistar rats, divided into six treatment groups (five rats per treatment), were used. At the expiration of administration, the rats were euthanized for the estimation of yeast translocation and haematological parameters. A total of 16 yeasts were isolated, out of which, five (Hanseniaspora uvarum, Hanseniaspora lachancei, Hanseniaspora opuntiae and two species of Kodamaea ohmeri) isolates that showed negative results for pathogenicity assays were used for in vitro and in vivo assays. The study revealed the growth of the test yeast strains at the different incubation temperatures (25-45 °C) and pH (4-9). However, significantly highest growths were observed at pH 4 and 5 and NaCl concentration of 4%. All the isolates showed remarkable growth at the bile concentrations used. In addition, the strains showed resistance to the antibiotics and antimycotics at the concentration used for investigation. In vivo assay results showed no significant differences between weight gain, haematological parameters and behavioural scores between control and treatment groups of the rats. The ability of the yeasts to show tolerance to stress conditions and lack of negative impacts to haematological, behavioural and yeast translocations are indications of their probiotic capabilities.
益生菌已被证明可改善宿主对维生素的摄取、产生维生素并改善整个胃肠道转运过程中的胃肠道功能，某些酵母被认为可能具有益生菌功能并带来一些健康益处。本研究的目的是评估从香蕉和大蕉果皮中分离出的酵母的益生菌潜力。使用标准程序从成熟的香蕉和大蕉果皮中分离酵母菌株。对致病性检测呈阴性的分离株用于体外和体内益生菌筛选。在体内试验中，使用了30只雄性Wistar大鼠，分为六个处理组（每组五只大鼠）。给药结束时，对大鼠实施安乐死以评估酵母易位和血液学参数。总共分离出16株酵母，其中五株（葡萄汁有孢汉逊酵母、拉氏有孢汉逊酵母、仙人掌有孢汉逊酵母和两株奥默柯达酵母）对致病性检测呈阴性的分离株用于体外和体内试验。研究揭示了受试酵母菌株在不同培养温度（25 - 45°C）和pH值（4 - 9）下的生长情况。然而，在pH值为4和5以及NaCl浓度为4%时观察到显著最高的生长。所有分离株在所用胆汁浓度下均表现出显著生长。此外，这些菌株在用于研究的浓度下对抗生素和抗真菌剂具有抗性。体内试验结果表明，大鼠对照组和处理组之间在体重增加、血液学参数和行为评分方面没有显著差异。酵母对压力条件表现出耐受性且对血液学、行为和酵母易位没有负面影响的能力表明它们具有益生菌能力。
