Wu Yi, Li Chen, Geng Zhiyuan, Wang Lihong, Wang Shufeng, Zhang Dingxing, Li Dongxu, Meng Fanyu
Harbin Medical University Public Health College, Harbin, 150081, China.
Probiotics Antimicrob Proteins. 2024 Dec 4. doi: 10.1007/s12602-024-10410-4.
Breast milk contains a diverse array of symbiotic microorganisms, including beneficial probiotics. While most research has focused on the bacterial composition of breast milk, the fungal community (mycobiota) has received limited attention. In this study, we isolated and evaluated yeast strains from breast milk, demonstrating the safety and probiotic potential of four strains through hemolysis tests, antimicrobial activity, tolerance to gastrointestinal conditions, aggregation, cell adhesion, and antioxidant activity assays. These yeast strains significantly inhibited the proliferation of pathogenic bacteria and showed strong antioxidant properties. Moreover, lysates from Rhodotorula mucilaginosa and Cryptococcus laurentii isolated from breast milk markedly inhibited the proliferation of colon cancer cell lines (HT-29 and SW620) and induced apoptosis by upregulating key apoptosis-related genes (caspase 3, caspase 9, and Bax), as well as autophagy-related genes (Beclin-1, LC3, and ATG5). This study provides valuable insights into the potential of breast milk-derived yeast strains for developing novel probiotic therapies for colon cancer treatment.
母乳中含有多种共生微生物，包括有益的益生菌。虽然大多数研究都集中在母乳的细菌组成上，但真菌群落（真菌群）受到的关注有限。在本研究中，我们从母乳中分离并评估了酵母菌株，通过溶血试验、抗菌活性、对胃肠道条件的耐受性、聚集、细胞粘附和抗氧化活性测定，证明了四株菌株的安全性和益生菌潜力。这些酵母菌株显著抑制了病原菌的增殖，并表现出强大的抗氧化特性。此外，从母乳中分离出的粘红酵母和罗伦隐球酵母的裂解物显著抑制了结肠癌细胞系（HT-29和SW620）的增殖，并通过上调关键的凋亡相关基因（半胱天冬酶3、半胱天冬酶9和Bax）以及自噬相关基因（Beclin-1、LC3和ATG5）诱导凋亡。本研究为母乳来源的酵母菌株开发用于结肠癌治疗的新型益生菌疗法的潜力提供了有价值的见解。
