Mogmenga Iliassou, Somda Marius Kounbèsiounè, Ouattara Cheik Amadou Tidiane, Keita Ibrahim, Dabiré Yérobessor, Diguță Camelia Filofteia, Toma Radu Cristian, Ezeogu Lewis I, Ugwuanyi Jerry O, Ouattara Aboubakar S, Matei Florentina
Laboratoire de Microbiologie et de Biotechnologies Microbiennes, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka 410001, Enugu State, Nigeria.
Microorganisms. 2023 Mar 21;11(3):802. doi: 10.3390/microorganisms11030802.
In recent years, research on yeasts as probiotics has gained more and more interest, which will allow the development of "new" products in the probiotics market. In this context, seventeen yeast strains isolated from , a traditional beer produced in Burkina Faso, were assessed for their probiotic attributes. The yeast identification was performed by molecular methods, including PCR-RFLP and 5.8S-ITS region sequencing. (14 strains) was the predominantly identified species, followed by (2 strains) and (1 strain). Except for , all yeast strains grew well at human temperature. The yeast strains showed high resistance when they were exposed to simulated gastrointestinal conditions. Auto-aggregation ability was between 70.20 ± 10.53% and 91.82 ± 1.96%, while co-aggregation with ranged from 24.92 ± 3.96% to 80.68 ± 9.53% and with Typhimurium from 40.89 ± 8.18% to 74.06 ± 7.94%. Furthermore, the hydrophobicity of isolated strains toward n-hexane was in the range from 43.17 ± 5.07% to 70.73 ± 2.42%. All yeast strains displayed high antioxidant capabilities, and the strains did not show hemolysis halos, such that they can be considered safe. Additionally, strains strongly inhibited the growth of foodborne pathogens. This is the first preliminary study to identify and characterize the yeast strains isolated from with interesting probiotic properties.
近年来，将酵母作为益生菌的研究越来越受到关注，这将促使益生菌市场开发出“新型”产品。在此背景下，对从布基纳法索生产的一种传统啤酒中分离出的17株酵母菌株进行了益生菌特性评估。通过包括PCR-RFLP和5.8S-ITS区域测序在内的分子方法进行酵母鉴定。（14株）是主要鉴定出的物种，其次是（2株）和（1株）。除了，所有酵母菌株在人体温度下生长良好。酵母菌株在模拟胃肠道条件下表现出高抗性。自聚集能力在70.20±10.53%至91.82±1.96%之间，而与的共聚集范围为24.92±3.96%至80.68±9.53%，与鼠伤寒沙门氏菌的共聚集范围为40.89±8.18%至74.06±7.94%。此外，分离菌株对正己烷的疏水性在43.17±5.07%至70.73±2.42%之间。所有酵母菌株都表现出高抗氧化能力，且这些菌株未显示溶血晕圈，因此可认为是安全的。此外，菌株强烈抑制食源性病原体的生长。这是首次对从具有有趣益生菌特性的中分离出的酵母菌株进行鉴定和表征的初步研究。
