利用细菌双杂交系统对集胞藻PCC 6803藻蓝蛋白单体中蛋白质-蛋白质相互作用的研究。
A study of the protein-protein interactions in the phycocyanin monomer from sp PCC 6803 using a bacterial two-hybrid system.
作者信息
Shao Simi, Zhao Longsheng, Li Pingyi, Su Hainan, Chen Xiulan, Zhang Yuzhong
机构信息
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China.
College of Marine Life Sciences, Ocean University of China, Qingdao 266237, China.
出版信息
Eng Microbiol. 2022 Apr 17;2(2):100019. doi: 10.1016/j.engmic.2022.100019. eCollection 2022 Jun.
Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications. In this study, we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin α and β subunits that form the phycocyanin monomer. Key amino acid residues responsible for the interaction between the subunits were identified, providing direct experimental evidence for the intramolecular interaction.
研究溶液中天然蛋白质的分子内相互作用对于理解其结构稳定性以及未来应用中的潜在用途非常重要。在本研究中，我们使用细菌双杂交系统来研究形成藻蓝蛋白单体的藻蓝蛋白α亚基和β亚基之间的相互作用。确定了负责亚基间相互作用的关键氨基酸残基，为分子内相互作用提供了直接的实验证据。
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