Wang Xingyan, Zhou Haibo, Ren Xiangmei, Chen Hanna, Zhong Lin, Bai Xianping, Bian Xiaoying
Helmholtz International Lab for Anti-Infectives, State Key Laboratory of Microbial Technology, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao 266237, China.
Eng Microbiol. 2023 Aug 2;3(3):100106. doi: 10.1016/j.engmic.2023.100106. eCollection 2023 Sep.
Iron is essential for bacterial survival, and most bacteria capture iron by producing siderophores. bacteria produce various types of bioactive secondary metabolites, such as ornibactin and malleobactin siderophores. In this study, the genome analysis of genomes showed a putative novel siderophore gene cluster , which is highly similar to the ornibactin and malleobactin gene clusters but does not have , a gene encoding a formyltransferase for N-‑hydroxy-ornithine formylation. Establishing the bacteriophage recombinase Redγ-Redαβ7029 mediated genome editing system in a non-model strain CICC 10960 allowed the rapid identification of the products of gene cluster, caribactins A-F (). Caribactins contain a special amino acid residue N-‑hydroxy-N--acetylornithine (haOrn), which differs from the counterpart N-‑hydroxy-N--formylornithine (hfOrn) in ornibactin and malleobactin, owing to the absence of . Gene inactivation showed that the acetylation of hOrn is catalyzed by CrbK, whose homologs probably not be involved in the biosynthesis of ornibactin and malleobactin, showing possible evolutionary clues of these siderophore biosynthetic pathways from different genera. Caribactins promote biofilm production and enhance swarming and swimming abilities, suggesting that they may play crucial roles in biofilm formation. This study also revealed that recombineering has the capability to mine novel secondary metabolites from non-model species.
铁对于细菌的生存至关重要，大多数细菌通过产生铁载体来捕获铁。细菌会产生各种类型的生物活性次生代谢产物，如鸟氨酸菌素和马勒菌素等铁载体。在本研究中，对[具体细菌名称未给出]基因组分析显示出一个推定的新型铁载体基因簇，它与鸟氨酸菌素和马勒菌素基因簇高度相似，但缺少一个编码用于N-羟基鸟氨酸甲酰化的甲酰基转移酶的基因。在非模式菌株CICC 10960中建立噬菌体重组酶Redγ-Redαβ7029介导的基因组编辑系统，使得能够快速鉴定该基因簇的产物，即加勒比菌素A-F（[此处可能有相关产物的具体说明未给出]）。加勒比菌素含有一个特殊的氨基酸残基N-羟基-N-乙酰鸟氨酸（haOrn），由于缺少[相关基因或酶]，它与鸟氨酸菌素和马勒菌素中的对应物N-羟基-N-甲酰鸟氨酸（hfOrn）不同。基因失活表明hOrn的乙酰化是由CrbK催化的，其同源物可能不参与鸟氨酸菌素和马勒菌素的生物合成，这显示了这些来自不同属的铁载体生物合成途径可能的进化线索。加勒比菌素促进生物膜的产生，并增强群体运动和游动能力，表明它们可能在生物膜形成中发挥关键作用。这项研究还表明重组工程有能力从非模式[细菌名称未给出]物种中挖掘新型次生代谢产物。
