Sun Xin, Zhang Haobin, Jia Yuping, Li Jingyi, Jia Meirong
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, NHC Key Laboratory of Biosynthesis of Natural Products, CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Shandong Academy of Pharmaceutical Sciences, Jinan 250101, China.
Eng Microbiol. 2024 May 28;4(3):100154. doi: 10.1016/j.engmic.2024.100154. eCollection 2024 Sep.
Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis of terpenoids in microorganisms offers an alternative and sustainable approach for efficient production. Various genome-editing technologies have been developed for microbial strain construction. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) is the most commonly used system owing to its outstanding efficiency and convenience in genome editing. In this review, the basic principles of CRISPR-Cas9 systems are briefly introduced and their applications in engineering bacteria for the production of plant-derived terpenoids are summarized. The aim of this review is to provide an overview of the current developments of CRISPR-Cas9-based genome-editing technologies in bacterial engineering, concluding with perspectives on the challenges and opportunities of these technologies.
萜类化合物被广泛用作药物、香料和生物燃料。然而，这些天然产物的使用在很大程度上受到其在天然植物中含量较低的限制。幸运的是，萜类化合物在微生物中的异源生物合成提供了一种高效生产的替代且可持续的方法。已开发出各种基因组编辑技术用于构建微生物菌株。成簇规律间隔短回文重复序列（CRISPR）-CRISPR相关蛋白9（Cas9）是最常用的系统，因为它在基因组编辑方面具有出色的效率和便利性。在本综述中，简要介绍了CRISPR-Cas9系统的基本原理，并总结了它们在工程菌生产植物源萜类化合物中的应用。本综述的目的是概述基于CRISPR-Cas9的基因组编辑技术在细菌工程中的当前发展情况，并对这些技术的挑战和机遇进行展望。
