Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2201711119. doi: 10.1073/pnas.2201711119. Epub 2022 Jul 11.
Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by linking CO capture and solar energy storage. As a typical methylotroph, shows great potential in methanol biotransformation. However, challenges still remain in engineering methanol metabolism for chemical overproduction. Here, we present the global rewiring of the central metabolism for efficient production of free fatty acids (FFAs; 23.4 g/L) from methanol, with an enhanced supply of precursors and cofactors, as well as decreased accumulation of formaldehyde. Finally, metabolic transforming of the fatty acid cell factory enabled overproduction of fatty alcohols (2.0 g/L) from methanol. This study demonstrated that global metabolic rewiring released the great potential of for methanol biotransformation toward chemical overproduction.
基于甲醇的生物炼制是一种很有前途的策略，可以通过连接 CO 捕获和太阳能存储来实现碳中和目标。作为一种典型的甲醇营养菌，在甲醇生物转化方面具有很大的潜力。然而，在工程甲醇代谢以进行化学过度生产方面仍然存在挑战。在这里，我们对中心代谢进行了全局重排，以有效地从甲醇生产游离脂肪酸（FFA；23.4 g/L），增加了前体和辅因子的供应，同时减少了甲醛的积累。最后，通过对脂肪酸细胞工厂进行代谢转化，使甲醇能够过量生产脂肪醇（2.0 g/L）。这项研究表明，全局代谢重排释放了甲醇生物转化的巨大潜力，使能够实现化学过度生产。
