Wei Hui, Wang Wei, Chou Yat-Chen, Himmel Michael E, Chen Xiaowen, Bomble Yannick J, Zhang Min
Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.
Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.
Eng Microbiol. 2023 Jan 10;3(2):100074. doi: 10.1016/j.engmic.2023.100074. eCollection 2023 Jun.
The decarbonization of the chemical industry and a shift toward circular economies because of high global CO emissions make CO an attractive feedstock for manufacturing chemicals. Moreover, H is a low-cost and carbon-free reductant because technologies such as solar-driven electrolysis and supercritical water (scHO) gasification enable sustainable production of molecular hydrogen (H). We review the recent advances in engineering , the representative species of "Knallgas" bacteria, for utilizing CO and H to autotrophically produce 2,3-butanediol (2,3-BDO). This assessment is focused on state-of-the-art approaches for splitting H to supply energy in the form of ATP and NADH to power cellular reactions and employing the Calvin-Benson-Bassham cycle for CO fixation. Major challenges and opportunities for application and future perspectives are discussed in the context of developing other promising CO and H-utilizing microorganisms, exemplified by .
由于全球二氧化碳排放量高，化学工业的脱碳以及向循环经济的转变使得一氧化碳成为制造化学品的有吸引力的原料。此外，氢气是一种低成本且无碳的还原剂，因为诸如太阳能驱动电解和超临界水（scH₂O）气化等技术能够可持续地生产分子氢（H₂）。我们综述了工程学方面的最新进展，“爆鸣气”细菌的代表性种类，利用一氧化碳和氢气自养生产2,3 - 丁二醇（2,3 - BDO）的情况。本评估聚焦于将氢气分解以ATP和NADH形式提供能量来驱动细胞反应的最新方法，以及采用卡尔文 - 本森 - 巴斯姆循环进行二氧化碳固定的方法。在开发其他有前景的利用一氧化碳和氢气的微生物（以……为例）的背景下，讨论了应用的主要挑战和机遇以及未来展望。
